Department of Ophthalmology (Y.G., D.L., X.W., K.L., H.Y., X.Z.), Xuanwu Hospital, Capital Medical University, Beijing, China.
Department of Biomedical Engineering, School of Biological Science and Medical Engineering (Y.G.), Beihang University, Beijing, China.
Stroke. 2020 Aug;51(8):2568-2572. doi: 10.1161/STROKEAHA.119.028809. Epub 2020 Jul 20.
The retina, as an externally located neural tissue, offers unique advantages in investigating the effect of therapeutic intervention on the brain. In this study, we put forth a clinically relevant model of retinal ischemia and reperfusion in nonhuman primates.
Acute retinal artery ischemia and reperfusion was induced by injecting an autologous clot into the ophthalmic artery of adult rhesus monkeys, and recanalization was achieved by focal thrombolysis with tPA (tissue-type plasminogen activator). Digital subtraction angiography and fluorescein angiography were used to evaluate blood flow in the retina and the choroid. Electroretinogram, optical coherence tomography, and hematoxylin and eosin staining were used to evaluate the structure and function of the retina after ischemia.
Digital subtraction angiography and fluorescein angiography images confirmed occlusion of the ophthalmic and central retinal arteries, as well as recanalization after tPA thrombolysis. Electroretinogram indicated retinal functional damage following ischemia, and thrombolysis partially rescued its impairment. Optical coherence tomography and hematoxylin and eosin staining revealed ischemia-induced changes in the retina, and tPA partially mitigated these damages.
This novel acute retinal artery ischemia and reperfusion model in rhesus monkeys may closely simulate retinal ischemia/reperfusion in clinical practice and provide an optimal platform for screening neuroprotective strategies.
视网膜作为一种位于外部的神经组织，在研究治疗干预对大脑的影响方面具有独特的优势。在本研究中，我们提出了一种在非人类灵长类动物中具有临床相关性的视网膜动脉缺血再灌注模型。
通过将自体血栓注入猴眼动脉来诱导急性视网膜动脉缺血和再灌注，并通过 tPA（组织型纤溶酶原激活物）进行局部溶栓来实现再通。数字减影血管造影和荧光素血管造影用于评估视网膜和脉络膜的血流。视网膜电图、光相干断层扫描和苏木精-伊红染色用于评估缺血后视网膜的结构和功能。
数字减影血管造影和荧光素血管造影图像证实了眼动脉和中央视网膜动脉的闭塞，以及 tPA 溶栓后的再通。视网膜电图表明缺血后视网膜功能损伤，溶栓部分挽救了其损伤。光相干断层扫描和苏木精-伊红染色显示缺血引起的视网膜变化，tPA 部分减轻了这些损伤。
这种新型的恒河猴急性视网膜动脉缺血再灌注模型可能密切模拟临床实践中的视网膜缺血/再灌注，并为筛选神经保护策略提供了一个最佳平台。
