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整合代谢组学-蛋白质组学分析揭示了葡萄糖胁迫对乳球菌乳亚种 CICC23200 代谢适应的影响。

Integrated metabonomic-proteomic analysis reveals the effect of glucose stress on metabolic adaptation of Lactococcus lactis ssp. lactis CICC23200.

机构信息

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science & Technology, Ministry of Education, Tianjin 300457, P.R. China; National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, P.R. China.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science & Technology, Ministry of Education, Tianjin 300457, P.R. China; National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; Tianjin Key Laboratory of Industrial Microbiology, Tianjin University of Science & Technology, Tianjin 300457, P.R. China; College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, P.R. China.

出版信息

J Dairy Sci. 2020 Sep;103(9):7834-7850. doi: 10.3168/jds.2019-17810. Epub 2020 Jul 16.

Abstract

A combined proteomic and metabonomic approach was used to investigate the metabolism of Lactococcus lactis ssp. lactis subjected to glucose stress treatment. A proteomic method was used to determine 1,427 altered proteins, including 278 proteins with increased expression and 255 proteins with decreased expression. A metabonomic approach was adopted to identify 98 altered metabolites, including 62 metabolites with increased expression and 26 metabolites with decreased expression. The integrated analysis indicated that the RNA and DNA mismatch repair process and energy metabolism were enhanced in response to high-glucose stress in L. lactis. Lactococcus lactis responded to glucose stress by up-regulating oxidoreductase activity, which acted on glycosyl bonds, hydrolase activity, and organic acid transmembrane transporter activity. This led to an improvement in the metabolic flux from glucose to pyruvate, lactate, acetate, and maltose. Down-regulation of amino acid transmembrane transporter, aminoacyl-transfer RNA ligase, hydroxymethyl-, formyl-, and related transferase activities resulted in a decrease in the nitrogen metabolism-associated metabolic pathway, which might be related to inhibition of the production of biogenic amines. Overall, we highlight the response of metabolism to glucose stress and provide potential possibilities for the reduced formation of biogenic amines in improved level of sugar in the dairy fermentation industry. Moreover, according to the demand for industrial production, sugar concentration in fermented foods should be higher, or lower, than a set value that is dependent on bacterial strain and biogenic amine yield.

摘要

采用蛋白质组学和代谢组学相结合的方法研究了乳糖乳球菌亚属乳糖受葡萄糖胁迫处理后的代谢变化。蛋白质组学方法检测到 1427 个差异表达蛋白,其中 278 个蛋白表达上调,255 个蛋白表达下调。代谢组学方法鉴定到 98 个差异代谢物,其中 62 个代谢物表达上调,26 个代谢物表达下调。综合分析表明,L. lactis 对高葡萄糖胁迫的反应增强了 RNA 和 DNA 错配修复过程和能量代谢。L. lactis 通过上调氧化还原酶活性、糖苷键作用水解酶活性和有机酸酸跨膜转运蛋白活性来应对葡萄糖胁迫。这导致从葡萄糖到丙酮酸、乳酸、乙酸和麦芽糖的代谢通量增加。氨基酸跨膜转运蛋白、氨酰-tRNA 连接酶、羟甲基、甲酰基和相关转移酶活性的下调导致与氮代谢相关的代谢途径减少,这可能与生物胺生成的抑制有关。总的来说,我们强调了代谢对葡萄糖胁迫的反应,并为减少乳制品发酵工业中生物胺的形成提供了潜在的可能性。此外,根据工业生产的需求,发酵食品中的糖浓度应高于或低于设定值,具体取决于细菌菌株和生物胺产量。

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