Bulut Arikan F, Özdemir F A, Şen D, Erdem S, Yörübulut S, Doğan H, Keskin L
Kırıkkale University, Faculty of Medicine, Dept. of Physiology, Elazig, Turkey.
Kırıkkale University, Faculty of Medicine, Bingol University, Faculty of Science and Art, Dept. of Molecular Biology and Genetics, Elazig, Turkey.
Acta Endocrinol (Buchar). 2020 Jan-Mar;16(1):15-21. doi: 10.4183/aeb.2020.15.
Thyroid disorders are common in diabetics and related to severe diabetic complications. TRPV2 ion channels have crucial functions in insulin secretion and glucose metabolism which have an important role in the pathophysiology of diabetes. Also, they have a significant effect on various immunological events that are involved in the HT pathophysiology.
This study aimed to investigate rs14039 and rs4792742 polymorphisms of the TRPV2 ion channels in type 2 diabetes mellitus (T2DM, n=100) Hashimoto thyroiditis (HT, n=70) and comorbid T2DM and HT (T2DM+HT, n=100) patients and control (n=100).
Case-control study.
RT-PCR genotyping was used to determine rs14039 and rs4792742 polymorphisms with DNA samples of subjects and appropriate primer and probes. Besides, required biochemical analyses were performed.
It was determined that the frequencies of the rs14039 GG homozygote polymorphic genotype and the G allele were significantly higher in T2DM+HT patients compared to the control (p=0.03 and p=0.01, respectively) and that especially the GG genotype increases the risk of T2DM+HT 3.046-fold (p=0.01, OR=3.046). It was detected that the GG genotype increased the risk of HT 2.54-fold (p=0.05, OR=2.541). TRPV2 rs4792742 polymorphisms reduce the risk of HT and T2DM+HT comorbidity almost by half and have a protective effect against HT and T2DM+HT.
The rs14039 GG genotype of the TRPV2 gene significantly increases the risks of development of T2DM+HT and HT disorders, may have a significant role in the pathophysiology of these diseases, also leading to predisposition for their development. Conversely, rs4792742 polymorphic genotypes have a strong protective effect against the HT and T2DM+HT comorbidity.
甲状腺疾病在糖尿病患者中很常见，且与严重的糖尿病并发症相关。瞬时受体电位香草酸亚型2（TRPV2）离子通道在胰岛素分泌和葡萄糖代谢中具有关键功能，这在糖尿病的病理生理学中起着重要作用。此外，它们对桥本甲状腺炎（HT）病理生理学中涉及的各种免疫事件也有显著影响。
本研究旨在调查TRPV2离子通道的rs14039和rs4792742多态性在2型糖尿病（T2DM，n = 100）、桥本甲状腺炎（HT，n = 70）、T2DM合并HT（T2DM + HT，n = 100）患者及对照组（n = 100）中的情况。
病例对照研究。
采用逆转录聚合酶链反应（RT-PCR）基因分型法，利用受试者的DNA样本以及合适的引物和探针来确定rs14039和rs4792742多态性。此外，还进行了所需的生化分析。
确定T2DM + HT患者中rs14039 GG纯合子多态性基因型和G等位基因的频率显著高于对照组（分别为p = 0.03和p = 0.01），尤其是GG基因型使T2DM + HT的风险增加3.046倍（p = 0.01，比值比[OR]=3.046）。检测发现GG基因型使HT的风险增加2.54倍（p = 0.05，OR = 2.541）。TRPV2 rs4792742多态性使HT和T2DM + HT合并症的风险几乎降低一半，对HT和T2DM + HT具有保护作用。
TRPV2基因的rs14039 GG基因型显著增加了T2DM + HT和HT疾病发生的风险，可能在这些疾病的病理生理学中起重要作用，也导致了其发病倾向。相反，rs4792742多态性基因型对HT和T2DM + HT合并症具有强大的保护作用。
