Lee M M, Schuessler G B, Lau K, Chien S
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
J Ultrastruct Mol Struct Res. 1988 Nov-Dec;101(2-3):236-42. doi: 10.1016/0889-1605(88)90014-6.
The purpose of the present experiment was to study the effects of internal hydrostatic pressure on vesicle size, density, and distribution in the canine carotid arterial endothelium by transmission electron microscopy. The pressures applied in this study were 0 (control), 40, 60, 80, 100, and 150 mm Hg. The results of transmission electron microscopy and computer analysis on the plasmalemmal vesicles of aortic endothelium showed that luminal, abluminal, and junctional vesicles all increased their diameter as the pressure was raised from 0 mm Hg, reaching a maximum at 80 mm Hg, and then decreased in size with further increases in pressure to 150 mm Hg. There was a significant difference in diameter among vesicles in different regions of the endothelium, with the diameter of luminal vesicles larger than those of abluminal and junctional vesicles. The densities of vesicles showed very little change from 0 to 80 mm Hg; but they increased markedly as the pressure was further raised from 80 to 150 mm Hg. These results indicate that pressure is an important mechanical factor governing the size and density of plasmalemmal vesicles in aortic endothelium.
本实验的目的是通过透射电子显微镜研究内部流体静压力对犬颈动脉内皮中囊泡大小、密度和分布的影响。本研究中施加的压力为0（对照）、40、60、80、100和150毫米汞柱。对主动脉内皮质膜囊泡进行的透射电子显微镜和计算机分析结果表明，随着压力从0毫米汞柱升高，腔面膜泡、基底面膜泡和连接膜泡的直径均增大，在80毫米汞柱时达到最大值，然后随着压力进一步升高至150毫米汞柱，其大小减小。内皮不同区域的囊泡直径存在显著差异，腔面膜泡的直径大于基底面膜泡和连接膜泡。从0到80毫米汞柱，囊泡密度变化很小；但随着压力从80毫米汞柱进一步升高至150毫米汞柱，囊泡密度显著增加。这些结果表明，压力是控制主动脉内皮质膜囊泡大小和密度的重要机械因素。
