Cane Karen A, Mayer Melinda, Lidgett Angela J, Michael Anthony J, Hamill John D
School of Biological Sciences, Monash University, Melbourne, Victoria 3800, Australia.
Biotechnology and Biological Sciences Research Council, Institute of Food Research, Norwich Research Park, Norwich NR2 2AN, UK.
Funct Plant Biol. 2005 May;32(4):305-320. doi: 10.1071/FP04008.
Synthesis of the wound-inducible alkaloid, nicotine, in roots of the allotetraploid species Nicotiana tabacum L. is strongly influenced by the presence of two non-allelic genes, A and B. Together, these loci affect baseline transcript levels of genes dedicated to secondary metabolism (e.g. PMT and A622) as well as genes with roles in separate areas of primary metabolism (e.g. ODC, ADC, SAMS - polyamines; QPT - pyridine nucleotide cycle). Experiments comparing high alkaloid variety NC 95 (AABB genotype) and near-isogenic low alkaloid N. tabacum variety LAFC 53 (aabb genotype) indicate that together, mutations in the A and B loci diminish, but do not ablate, the propensity of roots to increase transcript levels of genes involved in alkaloid metabolism after damage to aerial tissues or direct treatment with the wound hormone, methyl jasmonate. Accordingly, roots of aabb genotype can increase their nicotine content somewhat in response to these treatments.
在异源四倍体物种烟草（Nicotiana tabacum L.）的根中，创伤诱导型生物碱尼古丁的合成受到两个非等位基因A和B的强烈影响。这两个基因座共同影响参与次生代谢的基因（如PMT和A622）以及在初级代谢不同区域起作用的基因（如ODC、ADC、SAMS - 多胺；QPT - 吡啶核苷酸循环）的基线转录水平。比较高生物碱品种NC 95（AABB基因型）和近等基因低生物碱烟草品种LAFC 53（aabb基因型）的实验表明，A和B基因座的突变共同降低了，但并未消除，根在地上组织受损或直接用创伤激素茉莉酸甲酯处理后增加参与生物碱代谢基因转录水平的倾向。因此，aabb基因型的根在响应这些处理时可以稍微增加其尼古丁含量。
