Spokevicius Antanas V, Van Beveren Kim S, Bossinger Gerd
School of Forest and Ecosystem Science, The University of Melbourne, Water Street, Creswick, Vic. 3363, Australia.
Funct Plant Biol. 2006 Mar;33(2):133-139. doi: 10.1071/FP05176.
In an attempt to devise a method for the rapid creation of somatic transgenic wood sectors of sufficient size that would allow us to detect and analyse altered wood characteristics within them, we have explored the manual wounding and subsequent infection with Agrobacterium of dormant lateral buds in poplar. Following treatment and transformation with a 35S-GUS construct, frequent stable transformation was found in the form of distinct and specific GUS staining patterns in the outer cortex, cambial region (including primary and secondary xylem and phloem) and pith. Sector frequency and size were consistent with anatomical features of dormant lateral buds at the time of manual wounding and Agrobacterium-infection. The suitability of somatic sector analysis for functional genomic studies as well as for studies investigating pattern formation and the developmental fate of various cell-types within poplar stems is discussed.
为了设计一种方法，能够快速创建足够大的体细胞转基因木材区域，以便我们检测和分析其中改变的木材特性，我们探索了对杨树休眠侧芽进行人工创伤并随后用农杆菌感染的方法。在用35S - GUS构建体处理和转化后，在外皮层、形成层区域（包括初生和次生木质部及韧皮部）和髓中发现了频繁的稳定转化，表现为独特而特定的GUS染色模式。区域频率和大小与人工创伤和农杆菌感染时休眠侧芽的解剖特征一致。本文讨论了体细胞区域分析在杨树茎功能基因组研究以及研究各种细胞类型的模式形成和发育命运方面的适用性。
