Grun Christoph, Berger Susanne, Matthes Daniel, Mueller Martin J
Julius-von-Sachs-Institute for Biosciences, Pharmaceutical Biology, University of Wuerzburg, Julius-von-Sachs-Platz 2, D-97082 Würzburg, Germany.
Funct Plant Biol. 2007 Feb;34(1):65-71. doi: 10.1071/FP06205.
The formation of non-enzymatic oxylipins is catalysed by reactive oxygen species. Reactive oxygen species are produced in response to pathogen attack. In this study, the accumulation of non-enzymatically formed hydroxy fatty acids and F-phytoprostanes in leaves of Arabidopsis thaliana (L.) Heyhn upon infection with Pseudomonas syringae was investigated and compared with the accumulation of the enzymatically formed oxylipins jasmonic acid and 12-oxo-phytodienoic acid. Levels of all oxylipins increased after infection with a virulent and with an avirulent strain of P. syringae. Inoculation of the avirulent strain resulted in a biphasic accumulation with a first maximum around 5 h which was missing after inoculation of the virulent strain. Levels of free and esterified hydroxy fatty acids and F-phytoprostanes increased after pathogen treatment; however, esterified compounds were 30 times more abundant than free oxylipins. The increase of the free compounds occurred later than the increase of the esterified compounds suggesting that non-enzymatic lipid oxidation occurs predominantly in membranes from which oxidised lipids can be released.
非酶氧化脂质的形成由活性氧催化。活性氧是在病原体攻击时产生的。在本研究中，对拟南芥叶片在感染丁香假单胞菌后非酶形成的羟基脂肪酸和F-植物前列腺素的积累进行了研究，并与酶促形成的氧化脂质茉莉酸和12-氧代植物二烯酸的积累进行了比较。感染丁香假单胞菌的强毒株和无毒株后，所有氧化脂质的水平均升高。接种无毒株导致双相积累，第一个峰值出现在约5小时左右，而接种强毒株后则没有这个峰值。病原体处理后，游离和酯化羟基脂肪酸以及F-植物前列腺素的水平升高；然而，酯化化合物的含量比游离氧化脂质丰富30倍。游离化合物的增加比酯化化合物的增加出现得晚，这表明非酶脂质氧化主要发生在能够释放氧化脂质的膜中。
