Chen Po-Yu, Huang Tsai-Lien, Huang Hao-Jen
Department of Life Sciences, National Cheng Kung University, No. 1 University Road, 701 Tainan, Taiwan, ROC.
Funct Plant Biol. 2007 Dec;34(11):995-1001. doi: 10.1071/FP07163.
It is well known that small quantities of nickel (Ni) are essential for plant species, and higher concentrations of Ni retard plant growth. However, the molecular mechanisms responsible for the regulation of plant growth by Ni are not well understood. The aim of this study is to investigate the early signalling pathways activated by Ni on rice (Oryza sativa L.) root. We showed that Ni elicited a remarkable increase in myelin basic protein (MBP) kinase activities. By immunoblot and immunoprecipitation analyses, it is suggested that Ni-activated 40- and 42-kDa MBP kinases are mitogen-activated protein kinases (MAPKs). Pretreatment of rice roots with the antioxidant, glutathione (GSH), the phospholipase D (PLD) inhibitor, n-butanol, and the calmodulin and CDPK antagonist and W7 inhibited Ni-induced MAPK activation. These results suggest that various signalling components are involved in transduction of the Ni signal in rice roots.
众所周知，少量的镍（Ni）对植物物种至关重要，而较高浓度的镍会抑制植物生长。然而，镍调控植物生长的分子机制尚未完全清楚。本研究的目的是探究镍在水稻（Oryza sativa L.）根上激活的早期信号通路。我们发现镍能显著提高髓鞘碱性蛋白（MBP）激酶活性。通过免疫印迹和免疫沉淀分析表明，镍激活的40 kDa和42 kDa MBP激酶是丝裂原活化蛋白激酶（MAPKs）。用抗氧化剂谷胱甘肽（GSH）、磷脂酶D（PLD）抑制剂正丁醇、钙调蛋白和CDPK拮抗剂W7预处理水稻根，可抑制镍诱导的MAPK激活。这些结果表明，多种信号成分参与了水稻根中镍信号的转导。
