Bianchi Michele Wolfe, Damerval Catherine, Vartanian Nicole
Biologie Cellulaire et Moléculaire de l'Adaptation à la Sécheresse (EA 1615), Université Paris XII, 94010 Créteil, France.
Station de Génétique Végétale, UMR 320 INRA/UPS/INA-PG, La Ferme du Moulon, 91190 Gif-sur-Yvette, France.
Funct Plant Biol. 2002 Jan;29(1):55-61. doi: 10.1071/PP01113.
Ten proteins differentially regulated by progressive drought stress in Arabidopsis Columbia wild-type, axr1-3 and axr2-1auxin-insensitive mutants, were identified from internal amino acid microsequencing. These proteins fell into two categories: (i) stress-related proteins, known to be induced by rapid water stress via abscisic acid (ABA)-dependent or -independent pathways [late embryogenesis abundant (LEA)-like and heat shock cognate (HS) 70, respectively], or in response to pathogens or oxidative stress [β-1,3 glucanase (BG), annexin] and (ii) metabolic enzymes [glutamine synthetase (GS), fructokinase (Frk), caffeoyl-CoA-3-O-methyltransferase (CCoAOMT)]. The differential behaviour of these proteins highlighted a role for AXR2 and/or AXR1 in the regulation of their abundance during drought adaptation. In particular, reduced induction of RD29B, GS and annexin, and overexpression of BG2 were observed specifically in the axr1-3 mutant, which is dramatically affected in several ABA-dependent drought adaptive responses, such as drought rhizogenesis. Altogether these results indicate cross-talk between auxin- and ABA-signalling in Arabidopsis drought responses.
通过内部氨基酸微测序，在拟南芥哥伦比亚野生型、axr1 - 3和axr2 - 1生长素不敏感突变体中鉴定出10种受渐进干旱胁迫差异调节的蛋白质。这些蛋白质分为两类：（i）胁迫相关蛋白，已知通过脱落酸（ABA）依赖性或非依赖性途径由快速水分胁迫诱导产生（分别为晚期胚胎丰富蛋白（LEA）样和热休克同源蛋白（HS）70），或响应病原体或氧化应激（β - 1,3葡聚糖酶（BG）、膜联蛋白）；（ii）代谢酶（谷氨酰胺合成酶（GS）、果糖激酶（Frk）、咖啡酰辅酶A - 3 - O - 甲基转移酶（CCoAOMT））。这些蛋白质的差异行为突出了AXR2和/或AXR1在干旱适应过程中对其丰度调节的作用。特别是，在axr1 - 3突变体中特异性观察到RD29B、GS和膜联蛋白的诱导减少，以及BG2的过表达，该突变体在几种ABA依赖性干旱适应性反应（如干旱生根）中受到显著影响。总之，这些结果表明拟南芥干旱反应中生长素信号和ABA信号之间存在相互作用。
