Loyaga-Castillo Monica, Calla-Poma Roger D, Calla-Poma Rubén, Requena-Mendizabal Margarita F, Millones-Gómez Pablo A
Department of Dentistry, Universidad Católica Los Ángeles de Chimbote, Perú.
Department of Oral Rehabilitation, Universidad Nacional Mayor de San Marcos, Perú.
J Contemp Dent Pract. 2020 May 1;21(5):509-514.
The purpose of this study was to compare the antifungal activity of three concentrations of a hydroethanolic extract of the × peel against strain ATCC 10231.
The agar diffusion method was used, and the culture medium used was Sabouraud agar. Petri dishes were prepared with concentrations of 10, 30, and 50% of hydroethanolic extract of the × peel; nystatin was used as a positive control, and 96% ethanol was used as a negative control. After 24 hours of incubation, each plate was examined, and the diameters (mm) of the growth inhibition halos were measured around each well using a digital vernier caliper.
The results showed that the antifungal activity of the extract varied, depending on the concentration, as shown using analysis of variance (ANOVA; < 0.05). When comparing the different concentrations, it was found by Duncan test that the greatest activity was obtained at 50%.
It was concluded that the hydroethanolic extract of × at 50% exerted a greater antifungal effect on the strain of than did the extract at lower concentrations.
By knowing the antimicrobial effect of × , this substance can be effectively used in products aimed to cure candidiasis infection.
本研究旨在比较三种浓度的×果皮水乙醇提取物对ATCC 10231菌株的抗真菌活性。
采用琼脂扩散法,使用的培养基为沙保弱琼脂。制备含有10%、30%和50%×果皮水乙醇提取物的培养皿;制霉菌素用作阳性对照,96%乙醇用作阴性对照。孵育24小时后,检查每个平板,并用数字游标卡尺测量每个孔周围生长抑制圈的直径(mm)。
结果表明,提取物的抗真菌活性因浓度而异,方差分析(ANOVA;<0.05)显示了这一点。比较不同浓度时,通过邓肯检验发现,50%浓度时活性最高。
得出结论,50%的×水乙醇提取物对该菌株的抗真菌作用比低浓度提取物更强。
通过了解×的抗菌作用,该物质可有效地用于旨在治疗念珠菌感染的产品中。