Exercise and Immunometabolism Research Group, Department of Physical Education, Post-Graduation Program in Movement Sciences, State University of São Paulo (UNESP), Presidente Prudente, São Paulo, Brazil.
Immunometabolism Research Group, Department of Cell and Developmental Biology, University of São Paulo, São Paulo, Brazil.
Cytokine. 2020 Oct;134:155207. doi: 10.1016/j.cyto.2020.155207. Epub 2020 Jul 18.
The nuclear transcriptional factor peroxisome proliferator activated receptor alpha (PPARα) plays a role in regulating genes involved in lipid metabolism, adipogenesis and inflammation. We aimed to assess the role of PPARα on exercise-mediated locally produced cytokines in adipose fat deposits and skeletal muscle. C57BL/6 (WT) and PPARα knockout (PPARα) mice were examined. Each genotype was randomly subdivided into three groups: non-exercised, and euthanized 2 or 24 h after a moderate aerobic exercise session (run on a treadmill at 60% of maximum speed for 1 h). Fat content in gastrocnemius muscle and lipolytic activity in isolated adipose tissue from mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue were evaluated. In addition, Interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor α (TNF-α) and monocyte chemoattractant protein 1 (MCP-1) content were evaluated by ELISA. WT mice showed a maximum lipolysis rate, as well as higher IL-6, IL-10, and IL10/TNF-α ratio values 2 h post-exercise (RPAT only) compared with PPARα mice. Taken together, our data suggests that PPARα knockout mice exhibited reduced lipolysis and anti-inflammatory response in adipose tissue following exercise, PPARα appears to play an important role in immunomodulatory and lipolysis signaling after acute moderate exercise.
过氧化物酶体增殖物激活受体α（PPARα）是一种核转录因子，在调节脂质代谢、脂肪生成和炎症相关基因中发挥作用。我们旨在评估 PPARα 在运动介导的脂肪组织和骨骼肌局部产生细胞因子中的作用。使用 C57BL/6（WT）和 PPARα 敲除（PPARα）小鼠进行检测。每个基因型随机分为三组：不运动组，以及在中等有氧运动后 2 或 24 小时安乐死组（在跑步机上以 60%最大速度运动 1 小时）。评估比目鱼肌中的脂肪含量和肠系膜（MEAT）和腹膜后（RPAT）脂肪组织中分离的脂肪组织的脂解活性。此外，通过 ELISA 评估白细胞介素 6（IL-6）、白细胞介素 10（IL-10）、肿瘤坏死因子 α（TNF-α）和单核细胞趋化蛋白 1（MCP-1）的含量。与 PPARα 小鼠相比，WT 小鼠在运动后 2 小时（仅 RPAT）显示出最大的脂解率以及更高的 IL-6、IL-10 和 IL10/TNF-α 比值。总之，我们的数据表明，PPARα 敲除小鼠在运动后脂肪组织中的脂解和抗炎反应减少，PPARα 在急性中等强度运动后的免疫调节和脂解信号中似乎发挥重要作用。
