Laboratório de Ecofisiologia e Biotecnologia Agrícola, Programa de Pós-Graduação em Agronomia, Universidade Estadual de Londrina, Londrina, Brazil.
Boyce Thompson Institute, Ithaca, USA.
Sci Rep. 2020 Jul 21;10(1):12048. doi: 10.1038/s41598-020-68949-5.
Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3' RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (UF) and ripe fruits (RF) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs.
辣椒是全球最重要的园艺作物之一。炭疽病（炭疽菌属）是辣椒生产的主要限制因素，造成了巨大的损失。辣椒素是存在于辣椒果实中的一种倍半萜类植物抗毒素，可以增强植物的抗性。参与辣椒素生物合成的遗传机制仍知之甚少。在这项研究中，使用 3' RNA 测序方法开发了未成熟（UF）和成熟（RF）辣椒果实对 C. scovillei 感染的转录谱数据集。结果显示，UF 中 4845 个基因上调，4720 个基因下调，RF 中 2560 个基因上调，1762 个基因下调。选择了四个与辣椒素相关的基因进行 RT-qPCR 分析，两个 5-表阿里他汀合酶（CA12g05030、CA02g09520）和两个 5-表阿里他汀-1,3-双加氧酶基因（CA12g05070、CA01g05990）。CA12g05030 和 CA01g05990 基因在 RF 中对真菌感染表现出早期反应（接种后 24 小时），在 96 HPI 时的表达分别增加了 68 倍和 53 倍。在 UF 中，所有基因均表现出晚期反应，特别是 CA12g05030，与对照植物相比，在 96 HPI 时的表达增加了 700 倍。我们在这里首次提供了辣椒果实对炭疽病反应的高通量表达数据集，为未来的辣椒育种计划提供了帮助。
