Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, 75012 Paris, France.
INSERM, Sorbonne Université, Institut Pasteur, UMR_S 1120, 75012 Paris, France.
Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934.
Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
钙是一种第二信使，对于从代谢调节和细胞存活到囊泡释放和运动等多种细胞过程至关重要。目前直接操纵内源性钙信号的策略缺乏细胞和亚细胞特异性。我们介绍了 SpiCee，这是一种多功能的基因编码螯合剂，结合了低亲和性和高亲和性钙结合位点。这种清除剂使我们能够改变体外和体内单个细胞内源性钙信号和功能，并具有生化控制的亚细胞分辨率。SpiCee 为研究体内局部钙信号并直接操纵这种第二信使用于治疗用途铺平了道路。
