Faculty of Technology, Department of Polymer Engineering, Düzce University, Düzce, Turkey.
Faculty of Science, Department of Chemistry, Gazi University, Ankara, Turkey.
J Environ Sci Health B. 2020;55(11):951-958. doi: 10.1080/03601234.2020.1797425. Epub 2020 Jul 22.
In this study, new polymers containing amides (, and ) were synthesized by condensation reaction for qualitative identification of insecticides. The synthesized polymers, including amides were investigated by infrared spectroscopy (IR), scanning electron microscopy-energy dispersion X- ray spectrometry (SEM-EDX), and gel permeation chromatography (GPC). Then, acetylcholinesterase enzyme (AChE) was covalently immobilized on these polymers to improve properties (including activity, reusability, and storage stability). Accordingly, organophosphate (malathion, acephate, chlorpyrifos methyl) and carbamate (carbofuran, methiocarb, methomyl), which are used to prevent harmful organisms in some agricultural products were enzymatically determined based on their inhibitory activity on AChE.
在这项研究中，通过缩合反应合成了含有酰胺（、和）的新型聚合物，用于杀虫剂的定性鉴定。通过红外光谱（IR）、扫描电子显微镜-能量色散 X 射线光谱（SEM-EDX）和凝胶渗透色谱（GPC）对合成的聚合物（包括酰胺）进行了研究。然后，将乙酰胆碱酯酶（AChE）共价固定在这些聚合物上，以改善其性质（包括活性、可重复使用性和储存稳定性）。因此，根据对 AChE 的抑制活性，利用有机磷（马拉硫磷、乙酰甲胺磷、毒死蜱甲基）和氨基甲酸酯（克百威、涕灭威、灭多威）来对一些农产品中的有害生物进行酶法测定。
