Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Vasco de Quiroga 4871, Santa Fe, CP 05348, Mexico City, Mexico.
J Microbiol Biotechnol. 2020 Oct 28;30(10):1592-1596. doi: 10.4014/jmb.2004.04030.
The aerobic growth and metabolic performance of strains BL21 and W3110 were studied when the hemoglobin (VHb) was constitutively expressed in the chromosome. When VHb was expressed, acetate production decreased in both strains and was nearly eliminated in BL21. Transcriptional levels of the glyoxylate shunt genes decreased in both strains when VHb was expressed. However, higher transcription of the α-ketoglutarate dehydrogenase genes were observed for W3110, while for BL21 transcription levels decreased. VHb expression reduced the transcription of the cytochrome bo genes only in BL21. These results are useful for better selecting a production host.
当血红蛋白（VHb）在染色体上组成性表达时，研究了菌株 BL21 和 W3110 的需氧生长和代谢性能。当表达 VHb 时，两种菌株的乙酸盐产量都下降，在 BL21 中几乎被消除。当表达 VHb 时，两种菌株的乙醛酸支路基因的转录水平都降低。然而，对于 W3110，α-酮戊二酸脱氢酶基因的转录更高，而对于 BL21，转录水平降低。VHb 表达仅在 BL21 中降低细胞色素 bo 基因的转录。这些结果有助于更好地选择生产宿主。
