Kanamori M, Ibuki F, Doi H, Yoon C H, Yawata T
J Nutr Sci Vitaminol (Tokyo). 1977;23(2):153-9. doi: 10.3177/jnsv.23.153.
The effects of various additives on the reaction of rennin with kappa-casein were investigated by using carboxymethylcellulose. Both urea and sodium 1-anilino-8-naphthalenesulfonate effectively inhibited rennin action at concentrations larger than 2 M and 2 mM, respectively. These reagents, however, activated the enzyme action at the lower concentrations. Both alpha S-and beta-caseins had some ranges of concentrations in which the rennin reaction was activated. Calcium chloride had an inhibitory effect on the rennin action. Neither mercaptoethanol nor KCl had any appreciable effect on the enzymatic hydrolysis of kappa-casein. These results are analyzed in terms of the association and dissociation of kappa-casein due to the presence of these additives in the reaction solutions.
通过使用羧甲基纤维素研究了各种添加剂对凝乳酶与κ-酪蛋白反应的影响。尿素和1-苯胺基-8-萘磺酸钠分别在浓度大于2M和2mM时有效抑制凝乳酶的作用。然而，这些试剂在较低浓度下会激活酶的作用。αS-酪蛋白和β-酪蛋白都有一定的浓度范围，在这些范围内凝乳酶反应被激活。氯化钙对凝乳酶的作用有抑制效果。巯基乙醇和氯化钾对κ-酪蛋白的酶促水解均无明显影响。根据反应溶液中这些添加剂的存在导致κ-酪蛋白的缔合和解离对这些结果进行了分析。
