Department of Head and Neck Surgery, The Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
Department of Head and Neck Surgery, The Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
Biochem Biophys Res Commun. 2020 Aug 20;529(2):148-155. doi: 10.1016/j.bbrc.2020.05.178. Epub 2020 Jun 21.
Long noncoding RNAs (lncRNAs) have undergone a comprehensive study for their involvements in tumor treatments. The purpose of our study was to explore the biological effects and regulatory mechanisms of lncRNA LINC01194 (LINC01194) in laryngeal squamous cell carcinoma (LSCC). The levels of LINC01194 in 105 LSCC patients were detected by RT-qPCR. The diagnostic and prognostic value of LINC01194 in LSCC patients were statistically analyzed. The potential functions of LINC01194 in proliferation, apoptosis, and metastasis of LSCC cells were evaluated. The interaction among LINC01194, miR-655 and SOX18 was explored by bioinformatics analysis, luciferase reporter assays and biotinylated RNA pull-down. We found that the expression levels of LINC01194 were highly expressed in LSCC, which was negatively correlated with the clinical outcome of LSCC patients. The area under the ROC curve for LINC01194 was up to 0.8388. Functional assays indicated that LINC01194 knockdown distinctly inhibited LSCC cells proliferation, induced apoptosis, and also attenuated LSCC cells migration and invasion in vitro. Furthermore, we elucidated that LINC01194 promoted SOX18 expression in LSCC cells via functioning as a molecular sponge for miR-655. Overall, based on our findings, LINC01194 served as a tumor promoter and potentially represents a novel prognostic indicator and therapeutic target in LSCC.
长链非编码 RNA（lncRNA）在肿瘤治疗中的作用已经得到了全面的研究。我们的研究目的是探讨 lncRNA LINC01194（LINC01194）在喉鳞状细胞癌（LSCC）中的生物学作用和调控机制。通过 RT-qPCR 检测 105 例 LSCC 患者中 LINC01194 的水平。对 LINC01194 在 LSCC 患者中的诊断和预后价值进行统计学分析。评估 LINC01194 对 LSCC 细胞增殖、凋亡和转移的潜在功能。通过生物信息学分析、荧光素酶报告实验和生物素化 RNA 下拉实验探讨 LINC01194、miR-655 和 SOX18 之间的相互作用。我们发现 LINC01194 在 LSCC 中的表达水平较高，与 LSCC 患者的临床结局呈负相关。LINC01194 的 ROC 曲线下面积高达 0.8388。功能测定表明，LINC01194 敲低明显抑制 LSCC 细胞增殖，诱导凋亡，并在体外减弱 LSCC 细胞迁移和侵袭。此外，我们阐明了 LINC01194 通过作为 miR-655 的分子海绵促进 LSCC 细胞中 SOX18 的表达。总之，根据我们的研究结果，LINC01194 作为一种肿瘤促进物，可能代表 LSCC 中的一种新的预后指标和治疗靶点。
