Ruh Kathryn E, Heins Bradley J, Salfer Isaac J, Gardner Robert D, Stern Marshall D
Department of Animal Science, University of Minnesota, St. Paul, MN.
Department of Animal Science, The Pennsylvania State University, University Park, PA.
Transl Anim Sci. 2018 Apr 13;2(2):125-134. doi: 10.1093/tas/txy014. eCollection 2018 Apr.
The objective of this study was to compare warm-season annual grasses to cool-season perennial () grasses for ruminal nutrient digestibility and N metabolism in a dual-flow continuous culture fermentation system. Dietary treatments were 1) fresh alfalfa, 2) grasses and legumes, 3) brown-midrib sorghum-sudangrass (), and 4) teff grass from an organic dairy production system. Eight dual-flow continuous culture fermenters were used during two consecutive 10-d periods consisting of 7 d for stabilization followed by 3 d of sampling. Fermenter samples were collected on days 8, 9, and 10 for analysis of pH, NH3-N, and VFA. Apparent DM, OM, NDF, and ADF digestibility were on average lesser ( < 0.05) in CSP grasses and legumes and warm-season annual grasses compared with alfalfa. True DM and OM digestibility were lesser ( < 0.05) for CSP grasses and legumes and warm-season annual grasses compared with fresh alfalfa. Total VFA were not affected ( > 0.05) by forage. The NH3-N concentrations were highest ( < 0.05) with alfalfa compared with the other CSP grasses and legumes and warm-season annual grasses. CP digestibility was not affected ( > 0.05) by forage treatment. Flow of NH3-N was greatest ( < 0.05) for alfalfa, reflecting the greatest NH3-N concentration. Flow of total N was greatest ( < 0.05) for alfalfa, intermediate for teff, and lowest for CSP grasses and legumes and BMRSS. Flows of bacterial N, efficiency of bacterial N, non-NH3-N, and dietary N were not affected ( > 0.05) by forage source. Overall, fermentation of warm-season grasses was similar to the cool-season grasses and legumes which indicate dairy producers may use warm-season grasses without concerns about negative impact on rumen health.
本研究的目的是在双流连续培养发酵系统中,比较暖季一年生禾本科牧草与冷季多年生禾本科牧草对瘤胃养分消化率和氮代谢的影响。日粮处理包括:1)新鲜苜蓿;2)禾本科牧草和豆科牧草;3)褐色中脉高粱-苏丹草;4)来自有机奶牛生产系统的提夫草。在连续两个10天的周期内使用了8个双流连续培养发酵罐,其中7天用于稳定期,随后3天进行采样。在第8、9和10天采集发酵罐样品,用于分析pH值、氨氮和挥发性脂肪酸。与苜蓿相比,冷季多年生禾本科牧草和豆科牧草以及暖季一年生禾本科牧草的干物质、有机物、中性洗涤纤维和酸性洗涤纤维表观消化率平均较低(P<0.05)。与新鲜苜蓿相比,冷季多年生禾本科牧草和豆科牧草以及暖季一年生禾本科牧草的真干物质和真有机物消化率较低(P<0.05)。总挥发性脂肪酸不受牧草的影响(P>0.05)。与其他冷季多年生禾本科牧草和豆科牧草以及暖季一年生禾本科牧草相比,苜蓿的氨氮浓度最高(P<0.05)。粗蛋白消化率不受日粮处理的影响(P>0.05)。苜蓿的氨氮流量最大(P<0.05),这反映了其最高的氨氮浓度。苜蓿的总氮流量最大(P<0.05),提夫草居中,冷季多年生禾本科牧草和豆科牧草以及褐色中脉高粱-苏丹草最低。细菌氮流量、细菌氮效率、非氨氮和日粮氮流量不受牧草来源的影响(P>0.05)。总体而言,暖季牧草的发酵与冷季牧草和豆科牧草相似,这表明奶牛生产者可以使用暖季牧草,而不用担心对瘤胃健康产生负面影响。