在体人多能干细胞来源的肠类器官用于研究干细胞维持。

In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance.

机构信息

Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Department of Pediatrics, University of Cincinnati, Cincinnati, OH, USA.

出版信息

Methods Mol Biol. 2020;2171:201-214. doi: 10.1007/978-1-0716-0747-3_12.

DOI:10.1007/978-1-0716-0747-3_12

PMID:32705643

Abstract

Human intestinal organoids (HIOs), derived from pluripotent stem cells, are a new tool to gain insights in gastrointestinal development, physiology, and associated diseases. Herein, we present a method for renal transplantation of HIOs in immunocompromised mice and subsequent analysis to study intestinal epithelial cell proliferation. In addition, we describe how to generate enteroids from transplanted HIOs. The method highlights the specific steps to successful engraftment and provides insight into the study of human intestinal stem cells.

摘要

人肠类器官（HIOs）来源于多能干细胞，是深入了解胃肠道发育、生理学和相关疾病的新工具。在此，我们介绍了一种将 HIO 移植到免疫缺陷小鼠肾脏中，并随后进行分析以研究肠道上皮细胞增殖的方法。此外，我们还描述了如何从移植的 HIO 中生成类肠器官。该方法强调了成功植入的具体步骤，并深入了解了人类肠道干细胞的研究。