Department of Chemical Engineering, Yıldız Technical University, İstanbul, Turkey.
Department of Pediatric, Şişli Hamidiye Etfal Training and Research Hospital, İstanbul, Turkey.
Biomed Chromatogr. 2020 Dec;34(12):e4951. doi: 10.1002/bmc.4951. Epub 2020 Aug 6.
Quantitative determination of omega-6 and omega-3 polyunsaturated fatty acids in human plasma and urine with high accuracy and precision provides significant information to monitor the underlying etiology of several diseases. In this regard, liquid chromatography-mass spectrometry is a good choice owing to its great selectivity and sensitivity. Additionally, the hybrid quadrupole-time of flight-mass spectrometer systems provides easy identification of target compounds with superior mass measurements. In this study, an analytical method has been developed for simple, accurate and simultaneous determination of linoleic acid, arachidonic acid, docosahexaenoic acid and eicosapentaenoic acid in a short chromatographic analysis period. The developed method is suitable for the quantitative detection of these four compounds with detection limits ranging between 1.1-3.0 ng ml and its applicability was assessed in human urine and plasma samples. As a result, acceptable accuracy (between 83 and 111%) and good precision (<6%) were obtained for target compounds using matrix matching calibration strategy.
定量测定人血浆和尿液中的 ω-6 和 ω-3 多不饱和脂肪酸，可提供有关多种疾病潜在病因的重要信息。在这方面，由于液相色谱-质谱法具有高选择性和高灵敏度，因此是一个不错的选择。此外，混合四极杆-飞行时间质谱仪系统可轻松识别具有优越质量测量的目标化合物。在这项研究中，开发了一种分析方法，用于在短的色谱分析时间内简单、准确和同时测定亚油酸、花生四烯酸、二十二碳六烯酸和二十碳五烯酸。所开发的方法适用于这四种化合物的定量检测，检测限在 1.1-3.0ng/ml 之间，其适用性在人尿和血浆样品中得到了评估。结果表明，使用基质匹配校准策略，目标化合物的准确度（83%至 111%之间）和精密度（<6%）均令人满意。
