Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
Department of Parasitology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-572, Japan; Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Mol Biochem Parasitol. 2020 Sep;239:111299. doi: 10.1016/j.molbiopara.2020.111299. Epub 2020 Jul 21.
Proper targeting and secretion of lysosomal hydrolases are regulated by transporting receptors. Entamoeba histolytica, the enteric protozoan parasite responsible for human amebiasis, has a unique family of lysosomal hydrolase receptors, cysteine protease binding protein family, CPBF. CPBFs, consisting of 11 members with conserved domain organization, bind to a wide range of cargos including cysteine proteases and glycosidases, which are also known to be involved in pathogenesis of this parasite. In this study, we characterized one of CPBFs, CPBF2, which is involved in cell motility and extracellular matrix invasion. Unexpectedly, these roles of CPBF were not related to its cargo, α-amylase. This is the first demonstration that a putative hydrolase receptor is involved in cell motility and invasion in parasitic protozoa.
溶酶体水解酶的适当靶向和分泌受转运受体调节。溶组织内阿米巴原虫是一种肠道原生动物寄生虫，可引起人类阿米巴病，它具有独特的溶酶体水解酶受体家族——半胱氨酸蛋白酶结合蛋白家族（CPBF）。CPBF 由 11 个具有保守结构域组织的成员组成，与广泛的货物结合，包括半胱氨酸蛋白酶和糖苷酶，这些货物也已知与该寄生虫的发病机制有关。在这项研究中，我们对 CPBF2 进行了表征，CPBF2 参与细胞运动和细胞外基质侵袭。出乎意料的是，CPBF 的这些作用与其货物α-淀粉酶无关。这是首次证明假定的水解酶受体参与寄生原生动物的细胞运动和侵袭。
