Center for Advanced Studies in Olive Grove and Olive Oils, Department of Experimental Biology, Univ. Jaén, 23071 Jaén, Spain.
Department of Crop Protection, Institute for Sustainable Agriculture, Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC), Campus 'Alameda del Obispo', Avenida Menéndez Pidal s/n, 14004 Apartado, Córdoba, Spain.
Int J Mol Sci. 2020 Jul 22;21(15):5197. doi: 10.3390/ijms21155197.
is the etiological agent of Verticillium wilt of olive. The virulence of Defoliating isolates usually displays differences and high plasticity. This work studied whether an epigenetic mechanism was involved in this plasticity. An inverse correlation between virulence and DNA methylation of protein-coding genes was found. A set of 831 genes was selected for their highly consistent inverse methylation profile and virulence in the five studied isolates. Of these genes, ATP-synthesis was highly represented, which indicates that the more virulent D isolates are, the more energy requirements they may have. Furthermore, there were numerous genes in the protein biosynthesis process: genes coding for the chromatin structure, which suggests that epigenetic changes may also affect chromatin condensation; many transmembrane transporter genes, which is consistent with denser compounds, traffic through membranes in more virulent isolates; a fucose-specific lectin that may play a role in the attachment to plant cell walls during the host infection process; and pathogenic cutinases that facilitate plant invasion and sporulation genes for rapid spreading alongside plants. Our findings support the notion that differences in the virulence of the Defoliating isolates may be controlled, at least to some extent, by an epigenetic mechanism.
是橄榄黄萎病的病原体。落叶型分离物的毒力通常表现出差异和高度的可塑性。本研究探讨了这一可塑性是否涉及表观遗传机制。发现毒力与蛋白质编码基因的 DNA 甲基化呈负相关。在研究的五个分离物中，选择了一组 831 个具有高度一致的反向甲基化谱和毒力的基因。在这些基因中，ATP 合成被高度代表，这表明更具毒力的 D 分离物可能具有更高的能量需求。此外，在蛋白质生物合成过程中有许多基因：染色质结构的基因编码，这表明表观遗传变化也可能影响染色质凝聚；许多跨膜转运蛋白基因，这与更具毒力的分离物中更密集的化合物通过膜运输一致；一种岩藻糖特异性凝集素，它可能在宿主感染过程中附着到植物细胞壁上发挥作用；以及致病角质酶，促进植物入侵和孢子形成基因，以便与植物一起快速传播。我们的研究结果支持这样一种观点，即落叶型分离物毒力的差异可能至少在一定程度上受到表观遗传机制的控制。
