Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Functional Genomics and Proteomics Unity, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Nutrients. 2020 Jul 16;12(7):2100. doi: 10.3390/nu12072100.
Hazelnuts ( L.) have an important role in human nutrition and health. However, they are a common cause of food allergy. Due to hazelnut varietal diversity, variety-dependent differences in the IgE-binding properties may be suspected, which could allow therapeutic strategies based on the use of hypoallergenic varieties to induce desensitization. In a proteogenomic approach, we aimed to evaluate the allergenic potential of a genetically diverse set of hazelnuts (n = 13 varieties). Minor differences were found at the level of genes encoding important allergens, namely Cor a 8, Cor a 9, and Cor a 14. Nevertheless, IgE-reactivity was similar for all varieties using sera from seven allergic individuals. The predominant IgE-reactive proteins were Cor a 9 (100%) and Cor a 1.04 (60%), with the former being the most frequently identified by a two-dimensional gel electrophoresis (2-DE)-based proteomic approach. Therefore, it seems that the conventional exclusion diet will hold its ground for the time being.
榛子（L.）在人类营养和健康中具有重要作用。然而，它们也是食物过敏的常见原因。由于榛子品种多样性，可能怀疑存在与品种相关的 IgE 结合特性差异，这可以允许基于使用低致敏性品种来诱导脱敏的治疗策略。在蛋白质组学方法中，我们旨在评估一组遗传多样化的榛子（n = 13 个品种）的致敏潜力。在编码重要过敏原的基因水平上发现了细微差异，即 Cor a 8、Cor a 9 和 Cor a 14。然而，使用来自 7 名过敏个体的血清，所有品种的 IgE 反应性相似。主要的 IgE 反应性蛋白是 Cor a 9（100%）和 Cor a 1.04（60%），前者是最常通过基于二维凝胶电泳（2-DE）的蛋白质组学方法鉴定的。因此，目前看来，传统的排除饮食将暂时占据主导地位。
