Marler Thomas E
Western Pacific Tropical Research Center, College of Natural and Applied Sciences, University of Guam, Mangilao, GU 96923, USA.
Plants (Basel). 2020 Jul 17;9(7):905. doi: 10.3390/plants9070905.
Methods in sunfleck research commonly employ the use of experimental leaves which were constructed in homogeneous light. These experimental organs may behave unnaturally when they are challenged with fluctuating light. Photosynthetic responses to heterogeneous light and leaf macronutrient relations were determined for , , and leaves that were grown in homogeneous shade, heterogeneous shade, or full sun. The speed of priming where one light fleck increased the photosynthesis during a subsequent light fleck was greatest for the leaves grown in heterogeneous shade. The rate of induction and the ultimate steady-state photosynthesis were greater for the leaves that were grown in heterogeneous shade versus the leaves grown in homogeneous shade. The leaf mass per area, macronutrient concentration, and macronutrient stoichiometry were also influenced by the shade treatments. The amplitude and direction in which the three developmental light treatments influenced the response variables were not universal among the three model species. The results indicate that the historical practice of using experimental leaves which were constructed under homogeneous light to study leaf responses to fluctuating light may produce artifacts that generate dubious interpretations.
光斑研究中的方法通常采用在均匀光照下构建的实验叶片。当这些实验器官受到波动光照的挑战时，它们的表现可能不自然。测定了在均匀遮荫、不均匀遮荫或全日照条件下生长的[具体植物1]、[具体植物2]和[具体植物3]叶片对异质光的光合响应以及叶片大量营养素关系。对于在不均匀遮荫下生长的叶片，一个光斑在随后的光斑期间增加光合作用的引发速度最快。与在均匀遮荫下生长的叶片相比，在不均匀遮荫下生长的叶片的诱导速率和最终稳态光合作用更高。叶片单位面积质量、大量营养素浓度和大量营养素化学计量也受到遮荫处理的影响。三种发育光照处理影响响应变量的幅度和方向在这三个模式物种中并不普遍。结果表明，使用在均匀光照下构建的实验叶片来研究叶片对波动光照的响应这一历史做法可能会产生导致可疑解释的假象。
