Department of Environmental Biotechnology, Faculty of Energy and Environmental Engineering, Silesian University of Technology, 2A Akademicka St., 44-100, Gliwice, Poland.
Faculty of Building Services, Hydro and Environmental Engineering, Warsaw University of Technology, Nowowiejska Str. 20, 00-653, Warsaw, Poland.
BMC Microbiol. 2020 Jul 24;20(1):222. doi: 10.1186/s12866-020-01896-3.
The study aimed to evaluate the influence of the duration times of anaerobic phases on the bacterial biocenosis characterisation while denitrifying dephosphatation in the Integrated Fixed-Film Activated Sludge - Moving-Bed Sequencing Batch Biofilm Reactor (IFAS-MBSBBR). The experiment was conducted in a laboratory model. The study consisted of four series, which differed in terms of the ratio of the anaerobic phases. duration concerning the overall reaction time in the cycle. The anaerobic phases covered from 18 to 30% of the whole cycle duration. During the reactor performance that took 9 months, the influent and effluent were monitored by analysis of COD, TKN, NH-N, NO-N, NO-N, TP, PO-P, pH, alkalinity and the phosphorus uptake batch tests. Characterisation of the activated sludge and the biofilm biocenosis was based on fluorescent in situ hybridisation (identification of PAO and GAO) and the denaturing gradient gel electrophoresis patterns.
The organic compounds removal was high (more than 95.7%) independently of cycle configuration. The best efficiency for nitrogen (91.1%) and phosphorus (98.8%) removal was achieved for the 30% share of the anaerobic phases in the reaction time. Denitrifying PAO (DPAO) covered more than 90% of PAO in the biofilm and usually around 70% of PAO in the activated sludge. A substantial part of the polyphosphate accumulating organisms (PAO) community were Actinobacteria. The denitrifying dephosphatation activity was performed mainly by Accumulibacter phosphatis.
High nutrient removal efficiencies may be obtained in IFAS-MBSBBR using the denitrifying dephosphatation process. It was found that the length of anaerobic phases influenced denitrification and the biological phosphorus removal. The extension of the anaerobic phases duration time in the reaction time caused an increase in the percentage share of denitrifying PAO (DPAO) in PAO. The biocenosis of the biofilm and the activated sludge reveal different species patterns and domination of the EBPR community.
本研究旨在评估在一体化固定膜-移动床生物膜反应器（IFAS-MBSBBR）中反硝化除磷过程中，厌氧阶段持续时间对细菌生物群落特征的影响。实验在实验室模型中进行。该研究包括四个系列，它们在厌氧阶段与整个周期时间的比例方面有所不同。厌氧阶段占整个周期时间的 18%至 30%。在为期 9 个月的反应器性能测试中，通过分析 COD、TKN、NH-N、NO-N、NO-N、TP、PO-P、pH、碱度和磷吸收批试验来监测进水和出水。通过荧光原位杂交（PAO 和 GAO 的鉴定）和变性梯度凝胶电泳模式对活性污泥和生物膜生物群落进行了特征描述。
有机化合物的去除率很高（超过 95.7%），与循环配置无关。在反应时间中厌氧阶段占 30%时，氮（91.1%）和磷（98.8%）的去除效率最高。生物膜中的反硝化聚磷菌（DPAO）占 PAO 的比例超过 90%，而在活性污泥中通常占 PAO 的 70%左右。聚磷菌（PAO）群落的大部分是放线菌。反硝化除磷活性主要由聚磷菌（Accumulibacter phosphatis）完成。
在 IFAS-MBSBBR 中使用反硝化除磷工艺可以获得高的养分去除效率。结果表明，厌氧阶段的长度会影响反硝化和生物除磷。在反应时间内延长厌氧阶段的持续时间会导致反硝化聚磷菌（DPAO）在 PAO 中的比例增加。生物膜和活性污泥的生物群落显示出不同的物种模式和 EBPR 群落的优势。
