Expression of MHC class II antigens and immunoglobulins in immunized pig foetuses.

Pig foetal spleen, liver and thymus cells were examined by using polyclonal antibody to pig immunoglobulins and monoclonal antibody reacting with a light chain determinant of pig MHC class II antigens. Pig foetuses were immunized with flagellin on the 72nd day of prenatal life. On the 14th day following antigen administration, large numbers of class II antigen-bearing cells and Ig-containing cells were demonstrated in the spleen using the immunofluorescence technique. Topographical localization of these cells in cryostat sections was very similar.