State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
State Key Laboratory of Pollution Control and Resources Reuse, Shanghai Institute of Pollution Control and Ecological Security, College of Environmental Science and Engineering, Tongji University, Siping Road, Shanghai 200092, PR China.
Water Res. 2020 Oct 1;184:116197. doi: 10.1016/j.watres.2020.116197. Epub 2020 Jul 16.
Reject water contains complex components of organic compounds, which have significant influences on the nitrogen removal performance when treated using biological autotrophic nitrogen removal technology. In this study, a two-stage partial nitritation (PN)-anammox (floc-granule) system was established to treat reject water (COD/NH-N = 0.97 ± 0.15), and the evolution of organic compounds along PN and annamox bioreactors was investigated using gas chromatography-mass spectrometry and excitation-emission matrix. Also, the variation of PN and anammox sludge properties relating to COD reduction was examined. The PN-anammox system removed approximately 80% of total inorganic nitrogen and COD with hydraulic reaction time of 16 h. The influent organics (330-600 mg COD/L) in reject water were primarily composed of volatile, protein-like and humic acid-like organic compounds. PN process contributed 53 ± 18% of the overall COD removal, primarily including oxygen-containing organics (e.g. phenol), proteins and humic acids. Anammox process contributed 22 ± 15% of the overall COD removal, but large molecule acids (e.g. lactic acid) and small molecule alcohols (e.g. glycerol) were reoccurred, contributing to the effluent COD with recalcitrant hydrocarbons (e.g. n-Octadecane). Reject water increased the extracellular proteins/polysaccharides ratio of PN and anammox sludge, promoting the adsorption and degradation of organic compounds. High-throughput sequencing results showed that denitrifying bacteria of Ottowia increased from 0.03% to 14.4% in PN reactor, and of Denitratisoma increased from 9.6% to 15.4% in anammox reactor. The occurrence of these denitrifiers might mitigate the negative impact of organics to functional organisms. This study highlights the organics fate during PN-anammox treatment system, which is important to maintain the robust nitrogen removal when treating organics-containing and high ammonium concentration wastewater.
处理 reject 水（COD/NH-N=0.97±0.15）时， reject 水中含有复杂的有机化合物成分，对生物自养脱氮技术的氮去除性能有重大影响。在这项研究中，建立了两段式部分硝化（PN）-厌氧氨氧化（絮体-颗粒）系统来处理 reject 水，并用气相色谱-质谱联用仪和激发-发射矩阵研究了 PN 和厌氧氨氧化生物反应器中有机化合物的演变。此外，还研究了与 COD 降低有关的 PN 和厌氧氨氧化污泥性质的变化。PN-厌氧氨氧化系统在水力停留时间为 16 h 的情况下，去除了约 80%的总无机氮和 COD。reject 水中的进水有机物（330-600 mg COD/L）主要由挥发性、蛋白样和腐殖酸样有机化合物组成。PN 过程贡献了 53±18%的 COD 去除，主要包括含氧有机物（如苯酚）、蛋白质和腐殖酸。厌氧氨氧化过程贡献了 22±15%的 COD 去除，但大分子酸（如乳酸）和小分子醇（如甘油）再次出现，导致出水 COD 中存在难降解的碳氢化合物（如正十八烷）。reject 水增加了 PN 和厌氧氨氧化污泥的胞外蛋白/多糖比，促进了有机化合物的吸附和降解。高通量测序结果表明，PN 反应器中脱氮菌 Ottowia 从 0.03%增加到 14.4%，厌氧氨氧化反应器中 Denitratisoma 从 9.6%增加到 15.4%。这些脱氮菌的出现可能减轻了有机物对功能菌的负面影响。本研究强调了 PN-厌氧氨氧化处理系统中有机物的命运，这对于处理含有有机物和高氨氮浓度的废水时保持稳健的氮去除非常重要。
