Tuck Alex C, Bühler Marc
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
University of Basel, Basel, Switzerland.
Methods Mol Biol. 2021;2167:287-301. doi: 10.1007/978-1-0716-0716-9_16.
Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription . We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.
一些长链非编码RNA（lncRNA）基因编码功能性RNA产物，而其他一些则作为DNA元件或通过转录发挥作用。我们在此描述一种基于核酶的方法，用于在小鼠胚胎干细胞中耗尽内源性lncRNA，同时对其基因的干扰最小。这使得能够测试lncRNA产物的作用。
