利用自我切割核酶消耗长链非编码RNA
Long Non-coding RNA Depletion Using Self-Cleaving Ribozymes.
作者信息
Tuck Alex C, Bühler Marc
机构信息
Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland.
University of Basel, Basel, Switzerland.
出版信息
Methods Mol Biol. 2021;2167:287-301. doi: 10.1007/978-1-0716-0716-9_16.
PMID:32712926
Abstract
Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription . We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.
摘要
一些长链非编码RNA（lncRNA）基因编码功能性RNA产物，而其他一些则作为DNA元件或通过转录发挥作用。我们在此描述一种基于核酶的方法，用于在小鼠胚胎干细胞中耗尽内源性lncRNA，同时对其基因的干扰最小。这使得能够测试lncRNA产物的作用。
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