去甲肾上腺素灌注大鼠心脏中一条不寻常的磷脂酰肌醇代谢途径。
An unusual phosphatidylinositol turnover pathway in noradrenaline-perfused rat hearts.
作者信息
Woodcock E A, Smith A I, Wallace C A, White L B
机构信息
Monash University Department of Medicine, Prince Henry's Hospital, Melbourne, Victoria, Australia.
出版信息
Clin Exp Pharmacol Physiol. 1988 Apr;15(4):251-5. doi: 10.1111/j.1440-1681.1988.tb01067.x.
Abstract
- The phosphatidylinositol turnover pathway has been studied in noradrenaline-perfused rat hearts using anion-exchange high performance liquid chromatography. 2. The active calcium-releasing compound inositol-(1,4,5)trisphosphate was detected together with its degradation products inositol-(1,4)bisphosphate and inositol monophosphate. All these products increased in response to noradrenaline stimulation. 3. At noradrenaline perfusion times from 5 s to 20 min there was no appearance of inositol-(1,3,4,5)tetrakisphosphate or its degradation products: inositol-(1,3,4)trisphosphate or inositol-(1,3) and (3,4)bisphosphates. 4. These data suggest the absence of the inositol-(1,4,5)trisphosphate phosphorylation/dephosphorylation pathway in heart.
摘要
- 利用阴离子交换高效液相色谱法，对去甲肾上腺素灌注的大鼠心脏中的磷脂酰肌醇代谢途径进行了研究。2. 检测到活性钙释放化合物肌醇-（1,4,5）三磷酸及其降解产物肌醇-（1,4）二磷酸和肌醇单磷酸。所有这些产物在去甲肾上腺素刺激下均增加。3. 在去甲肾上腺素灌注时间从5秒到20分钟的过程中，未出现肌醇-（1,3,4,5）四磷酸或其降解产物：肌醇-（1,3,4）三磷酸或肌醇-（1,3）和（3,4）二磷酸。4. 这些数据表明心脏中不存在肌醇-（1,4,5）三磷酸磷酸化/去磷酸化途径。
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