Department of Animal Science, Faculty of Agricultural and Natural Resources, Persian Gulf University, Bushehr, Iran.
Department of Animal Science, Faculty of Agriculture, Sanandaj Branch, Islamic Azad University, Sanandaj, Kurdistan province, Iran.
J Therm Biol. 2020 Jul;91:102647. doi: 10.1016/j.jtherbio.2020.102647. Epub 2020 Jun 16.
Honey bees produce heat shock proteins (HSPs) following biotic and abiotic stressors to protect cells from damage. In the current study, the pattern of HSP70 gene transcription, foraging, and temperature inside and outside the hive and their association in Apis mellifera meda colonies during Ziziphus blooming period were investigated. Therefore, the number of bees entering the hive, temperature inside and outside the hive were recorded in six colonies at different times of the day. Entering and exiting worker bees were sampled in the front of three hives at three times during the day, morning, midday, and afternoon to evaluate HSP70 gene expression by real-time PCR. The results showed that the flight behavior was influenced by the inside and outside hive temperatures, which was lower and higher in the midday and at the end of the day, respectively. The peak amount of HSP70 gene transcription at the midday was associated with the lowest bee flight activity and highest inside and outside the hive temperatures. In addition, the peak of flight activity was associated with intermediate levels of HSP70 gene expression in the afternoon.
蜜蜂在受到生物和非生物胁迫后会产生热休克蛋白（HSPs），以保护细胞免受损伤。在当前的研究中，调查了在枣花期间，蜜蜂蜜蜂 meda 种群中 HSP70 基因转录、觅食和蜂巢内外温度的模式及其相关性。因此，在一天中的不同时间记录了六个蜂群中进入蜂巢的蜜蜂数量、蜂巢内外的温度。在白天的三个时间点，早上、中午和下午，从三个蜂巢的前面采集进出工蜂的样本，通过实时 PCR 评估 HSP70 基因表达。结果表明，飞行行为受到蜂巢内外温度的影响，中午和一天结束时的温度分别较低和较高。中午 HSP70 基因转录量最高与蜜蜂飞行活动最低以及蜂巢内外温度最高有关。此外，下午飞行活动的高峰期与 HSP70 基因表达的中间水平有关。
