Jackowski G, Suria D, Liew C C
Can J Biochem. 1976 Jan;54(1):9-14. doi: 10.1139/o76-002.
Isolation of nucleolar proteins was obtained by dissociation in the presence of urea-guanidine hydrochloride, followed by high-speed centrifugation to remove nucleic acids. At least 31 fractions of nucleolar proteins were detected by isoelectrofocusing gel electrophoresis in pH range 3.5-10. Following two-dimensional gel electrophoresis on sodium dodecyl sulfate-polyacrylamide slab gels, more than 100 components of nucleolar proteins were identifieid. Two-thirds of nucleolar proteins were located in the pH range 5-8 following isoelectrofocusing. The molecular weights of these classes of proteins were shown to be mostly 30000-70000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
在盐酸尿素-胍存在的情况下通过解离获得核仁蛋白，然后进行高速离心以去除核酸。通过在pH 3.5 - 10范围内的等电聚焦凝胶电泳检测到至少31个核仁蛋白组分。在十二烷基硫酸钠-聚丙烯酰胺平板凝胶上进行二维凝胶电泳后，鉴定出100多种核仁蛋白组分。等电聚焦后，三分之二的核仁蛋白位于pH 5 - 8范围内。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，这些类别的蛋白质分子量大多为30000 - 70000。
