School of Agriculture and Food Science and UCD Earth Institute, University College Dublin, Belfield, Dublin 4, Ireland.
Department of Crop Science, Teagasc Crops Environment and Land Use Programme, Teagasc, Oak Park, County Carlow, Ireland.
Phytopathology. 2021 Feb;111(2):356-368. doi: 10.1094/PHYTO-02-20-0041-R. Epub 2021 Jan 18.
Septoria tritici blotch (STB) is an important foliar disease of wheat that is caused by the fungal pathogen . The grass has been used previously as a model system for cereal-pathogen interactions. In this study, we examined the nonhost resistance (NHR) response of to two different isolates in comparison with wheat. These isolates vary in aggressiveness on wheat cultivar Remus, displaying significant differences in disease and pycnidia coverage. Using microscopy, we found that similar isolate-specific responses were observed for hydrogen peroxide accumulation and cell death in both wheat and . Despite this, induction of isolate-specific patterns of defense gene expression by did differ between and wheat. Our results suggest that expression of the phenylalanine ammonia lyase gene may be important for NHR in , while pathogenesis-related genes and expression of genes regulating reactive oxygen species may be important to limit disease in wheat. Future studies of the - interaction may allow identification of conserved plant immunity targets that are responsible for the isolate-specific responses observed in both plant species.
小麦壳针孢叶斑病（STB）是一种重要的小麦叶部病害，由真菌病原体引起。该草曾被用作禾本科病原菌相互作用的模式系统。在这项研究中，我们研究了与小麦相比，对两种不同的 分离株的非寄主抗性（NHR）反应。这些分离株在小麦品种雷穆斯上的侵袭力不同，在疾病和分生孢子覆盖率上存在显著差异。通过显微镜观察，我们发现，在小麦和 中均观察到了类似的与分离株特异性相关的过氧化氢积累和细胞死亡反应。尽管如此， 对防御基因表达的诱导模式与小麦不同。我们的研究结果表明，苯丙氨酸解氨酶 基因的表达可能对 中的 NHR 很重要，而与病程相关的 基因和调节活性氧的基因的表达可能对限制小麦病害很重要。对 相互作用的进一步研究可能有助于确定负责两种植物中观察到的与分离株特异性相关反应的保守植物免疫靶标。
