Adsorption of (Phe-h)/(Phe-d)-substituted peptides from neurotensin family on the nanostructured surfaces of Ag and Cu: SERS studies.

This work describes an application of Raman (RS) and surface-enhanced Raman scattering (SERS) to characterize the selective adsorption of two peptides belonging to the neurotensin family peptides, such as kinetensin (KN) and xenopsin-related peptide 2 (XP-2) that are known to stimulate the growth of human tumors. To perform a reliable analysis of SERS spectra, the L-Phe residue (at position 8 or 1 in the amino acid sequence of these peptides) was replaced with L-Phe-d (five protons of L-phenylalanine ring substituted by deuterium). Native and (Phe-d)-isotopically labeled peptides were deposited on electrochemically nanostructured surfaces of Ag (AgORC) and Cu (CuORC) from an aqueous solution (HO). To determine the share of amide bonds in the interaction with the metallic substrate, SERS spectra of peptides adsorbed on AgORC from heavy water (DO) were measured. Also, to determine the effect of the C-end on the SERS spectrum, measurements were made for the KN analog in which the C-terminal L-leucine was removed ([desLeu]KN). Based on the analyses of the spectral profiles, in the spectral range of 600-1650 cm, specific conclusions have been drawn regarding specific aromatic ring···metal interactions and changes in the interaction during substrate change.