Podstawka Edyta, Proniewicz Leonard M
Regional Laboratory of Physicochemical Analysis and Structural Research, Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Krakow, Poland.
J Phys Chem B. 2009 Apr 9;113(14):4978-85. doi: 10.1021/jp8110716.
We used surface-enhanced Raman scattering (SERS) to characterize the adsorption behavior of bombesin (BN) and five BN-related peptides, including phyllolitorin, [Leu(8)]phyllolitorin, neuromedin C (NMC), neuromedin B (NMB), and PG-L (Pseudophryne guntheri), in a silver colloidal solution. Our experiments show that the pyrrole coring of the Trp and aromatic ring of Phe of these peptides are preferentially adsorbed on silver nanoparticles. However, the geometry of the rings and the strength of the interactions with this surface vary among peptides. Additionally, these peptides are weakly coordinated to the colloidal silver surface through the CO fragment of a peptide bond, between Gln/Leu/His and Trp residues, and CNC and SC fragments. Also, using the recently reported SERS spectra of these peptides immobilized onto an electrochemically roughened silver electrode surface, we demonstrate substrate-induced changes in the adsorption behavior of these peptides. Comparative analysis indicates that the interactions between peptides and the enhancing surfaces depend strongly on the geometry of the Trp, CONH, and SC fragments of these biomolecules etched on the surfaces.
我们使用表面增强拉曼散射（SERS）来表征铃蟾肽（BN）以及包括叶泡蛙肽、[亮氨酸（8）]叶泡蛙肽、神经介素C（NMC）、神经介素B（NMB）和PG-L（冈氏拟姬蛙）在内的五种与BN相关的肽在银胶体溶液中的吸附行为。我们的实验表明，这些肽的色氨酸的吡咯环和苯丙氨酸的芳香环优先吸附在银纳米颗粒上。然而，这些环的几何形状以及与该表面相互作用的强度在不同肽之间有所不同。此外，这些肽通过肽键的CO片段、在谷氨酰胺/亮氨酸/组氨酸和色氨酸残基之间以及CNC和SC片段与胶体银表面形成弱配位。而且，利用最近报道的这些肽固定在电化学粗糙化银电极表面的SERS光谱，我们证明了底物诱导的这些肽吸附行为的变化。对比分析表明，肽与增强表面之间的相互作用强烈依赖于蚀刻在表面上的这些生物分子的色氨酸、CONH和SC片段的几何形状。
