Bonner T I, Young A C, Brann M R, Buckley N J
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20982.
Neuron. 1988 Jul;1(5):403-10. doi: 10.1016/0896-6273(88)90190-0.
The human and rat genes for a fifth muscarinic receptor have been cloned and expressed in mammalian cells. The 532 amino acid human protein has 89% sequence identity to the 531 amino acid rat protein and is most closely related to the m3 receptor. Both proteins are encoded by single exons. The receptor has intermediate affinity for pirenzepine and low affinity for AF-DX 116, and it increases metabolism of phosphatidylinositol when stimulated with carbachol. Expression of mRNA has yet to be observed in brain or selected peripheral tissues, suggesting that either it is substantially less abundant than m1-m4 or its distribution is quite different.
第五种毒蕈碱受体的人类和大鼠基因已被克隆并在哺乳动物细胞中表达。由532个氨基酸组成的人类蛋白质与由531个氨基酸组成的大鼠蛋白质具有89%的序列同一性，并且与m3受体关系最为密切。这两种蛋白质均由单个外显子编码。该受体对哌仑西平具有中等亲和力，对AF-DX 116具有低亲和力，在用卡巴胆碱刺激时会增加磷脂酰肌醇的代谢。在大脑或选定的外周组织中尚未观察到mRNA的表达，这表明要么它的丰度远低于m1-m4，要么其分布有很大不同。
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