Heydari Fariba, Abolafia Joaquín, Pedram Majid
Department of Plant Pathology , Faculty of Agriculture , Tarbiat Modares University , Tehran, Iran.
Departamento de Biología Animal , Biología Vegetal y Ecología , Universidad de Jaén, Campus Las Lagunillas , s/n, 23071, Jaén , Spain.
J Nematol. 2020;52:1-13. doi: 10.21307/jofnem-2020-062.
n. sp. is described and illustrated based upon its morphological, morphometric, and molecular characters. The new species is mainly characterized by its small body size (367-454 µm long females and 350, 391 µm long males) and has small lateral vulval flaps. It is further characterized by having six lines in the lateral fields, cephalic region slightly expanded, narrower adjacent to the body, short 6 to 7 µm long stylet with three posteriorly sloped knobs, no post-vulval uterine sac (PUS), conical tail, narrowing to a rounded tip, and rare males with slender tylenchoid spicules and bursa reaching the tail tip. By having six lines in the lateral fields, the new species was compared with five known species of the genus, namely , , and , having comparable number (six or seven) of lines in the lateral fields. It was further compared with having lateral vulval flaps. The differences with above-mentioned species are discussed. In molecular phylogenetic analyses using nearly full length small and large subunit ribosomal DNA (SSU and LSU D2-D3 rDNA) and cytochrome c oxidase subunit I ( mtDNA) gene sequences, n. sp. formed clade with species of the genus with different clade support values in Bayesian inference. n. sp. is described and illustrated based upon its morphological, morphometric, and molecular characters. The new species is mainly characterized by its small body size (367-454 µm long females and 350, 391 µm long males) and has small lateral vulval flaps. It is further characterized by having six lines in the lateral fields, cephalic region slightly expanded, narrower adjacent to the body, short 6 to 7 µm long stylet with three posteriorly sloped knobs, no post-vulval uterine sac (PUS), conical tail, narrowing to a rounded tip, and rare males with slender tylenchoid spicules and bursa reaching the tail tip. By having six lines in the lateral fields, the new species was compared with five known species of the genus, namely , , and , having comparable number (six or seven) of lines in the lateral fields. It was further compared with having lateral vulval flaps. The differences with above-mentioned species are discussed. In molecular phylogenetic analyses using nearly full length small and large subunit ribosomal DNA (SSU and LSU D2-D3 rDNA) and cytochrome c oxidase subunit I ( mtDNA) gene sequences, n. sp. formed clade with species of the genus with different clade support values in Bayesian inference.
基于其形态、形态测量和分子特征对新物种进行了描述和图示。新物种的主要特征是体型小（雌性体长367 - 454微米，雄性体长350、391微米），有小的侧阴门瓣。其进一步特征包括侧区有六条线，头部区域稍膨大，靠近身体处较窄，有6至7微米长的短口针，具三个向后倾斜的瘤，无阴门后子宫囊（PUS），尾部呈圆锥形，向圆形尖端逐渐变窄，罕见雄性具有细长的类垫刃线虫交合刺且交合伞到达尾尖。通过侧区有六条线，将新物种与该属五个已知物种进行了比较，即 、 、 和 ，它们在侧区有相当数量（六条或七条）的线。还将其与有侧阴门瓣的 进行了比较。讨论了与上述物种的差异。在使用近乎全长的小亚基和大亚基核糖体DNA（SSU和LSU D2 - D3 rDNA）以及细胞色素c氧化酶亚基I（线粒体DNA）基因序列进行的分子系统发育分析中，新物种在贝叶斯推断中与该属的物种形成了具有不同分支支持值的分支。基于其形态、形态测量和分子特征对新物种进行了描述和图示。新物种的主要特征是体型小（雌性体长367 - 454微米，雄性体长350、391微米），有小的侧阴门瓣。其进一步特征包括侧区有六条线，头部区域稍膨大，靠近身体处较窄，有6至7微米长的短口针，具三个向后倾斜的瘤，无阴门后子宫囊（PUS），尾部呈圆锥形，向圆形尖端逐渐变窄，罕见雄性具有细长的类垫刃线虫交合刺且交合伞到达尾尖。通过侧区有六条线，将新物种与该属五个已知物种进行了比较，即 、 、 和 ，它们在侧区有相当数量（六条或七条）的线。还将其与有侧阴门瓣的 进行了比较。讨论了与上述物种的差异。在使用近乎全长的小亚基和大亚基核糖体DNA（SSU和LSU D2 - D3 rDNA）以及细胞色素c氧化酶亚基I（线粒体DNA）基因序列进行的分子系统发育分析中，新物种在贝叶斯推断中与该属的物种形成了具有不同分支支持值的分支。
