Henan Academy of Agricultural Sciences, Institute of Animal Husbandry and Veterinary Science, Zhengzhou, Henan, People's Republic of China.
College of Animal Science and Technology, Northwest A&F University, Yangling, People's Republic of China.
Anim Biotechnol. 2022 Apr;33(2):273-278. doi: 10.1080/10495398.2020.1793769. Epub 2020 Jul 29.
Copy number variation mainly refers to the copy number change of DNA fragments from 1 to 5 Mb. The deletion, duplication, inversion and ectopic of these fragments are collectively referred to as CNV. Numerous studies have shown that transfer factors play a vital role in regulating the growth and development of the body, for example the pleomorphic adenoma gene (PLAG). However, there is no study of CNV in gene. We qualified copy numbers within gene in 8 cattle breeds (Qinchuan, Qaidamu, Jinjiang, Guangfeng, Ji'an, Jiaxian, Pinan and Xianan cattle) by quantitative PCR, and explored their impacts on CNV of gene and phenotypic traits in Xianan cattle. We defined Deletion into CN = 0, Normal into CN = 1 and Duplication into CN = 2. The results showed that the individual with type of CN = 1 has a significant better effect on heart girth in JA cattle population ( < 0.01); the individual with type of CN = 1 and CN = 0 has a better effect on Rump length in JX cattle population ( < 0.05); the individual with type of CN = 0 has a better effect on cannon bone circumference in XN cattle population ( < 0.05). Association analysis showed that in JA cattle, the number of CN = 2 is great in JA cattle population, and the performance of CN = 2 in heart girth is better than CN = 1; in JX cattle, the rump length of CN = 2 is less than individual with CN = 0 and CN = 1; in XN cattle, individuals with CN = 0 have a better performance on cannon bone circumference than others. The results can provide a theoretical basis for molecular breeding of Chinese cattle, molecular mark-assist selection (MAS) of growth traits of Chinese cattle, and rapidly establish a Chinese cattle population with excellent genetic resources. Simple summaryWith the living standards rising, people's demand for beef is getting higher and higher, and there is a great significance to improve the growth performance of cattle. We measured body size data and detected copy number type of different cattle breeds (Xianan cattle, Ji'an cattle and Jiaxian cattle), and analyzed the correlation between the two object. We found that copy number variation of gene significantly affected some growth traits of XN cattle, JA cattle, and JX cattle. This may provide the basic material for molecular marker-assisted selection breeding of Chinese cattle breeds.
拷贝数变异主要是指 DNA 片段从 1 到 5 Mb 的拷贝数变化。这些片段的缺失、重复、倒位和异位统称为 CNV。大量研究表明，转移因子在调节身体的生长和发育中起着至关重要的作用，例如多形性腺瘤基因（PLAG）。然而，目前还没有关于 基因 CNV 的研究。我们通过定量 PCR 对 8 个牛品种（秦川牛、柴达木牛、晋江牛、广丰牛、吉安牛、佳县牛、坪山牛和西安牛）中的 基因进行了拷贝数鉴定，并探讨了其对西安牛基因 CNV 和表型特征的影响。我们将缺失定义为 CN = 0，正常为 CN = 1，重复为 CN = 2。结果表明，JA 牛群中 CN = 1 个体的胸围效果显著更好（ < 0.01）；JX 牛群中 CN = 1 和 CN = 0 个体的臀部长度效果更好（ < 0.05）；XN 牛群中 CN = 0 个体的炮骨周长效果更好（ < 0.05）。关联分析表明，在 JA 牛群中，JA 牛的 CN = 2 数量较多，CN = 2 的胸围表现优于 CN = 1；在 JX 牛群中，CN = 2 的臀部长度小于 CN = 0 和 CN = 1 的个体；在 XN 牛群中，CN = 0 的个体的炮骨周长表现优于其他个体。该结果可为中国牛的分子育种、中国牛生长性状的分子标记辅助选择（MAS）以及快速建立具有优良遗传资源的中国牛群体提供理论依据。
