Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil.
Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455970, Brazil.
Bioprocess Biosyst Eng. 2020 Dec;43(12):2253-2268. doi: 10.1007/s00449-020-02411-8. Epub 2020 Jul 28.
In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol-ethylenediamine-glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic strength and in absence or presence of 0.5% (v/v) Triton X-100). The biocatalysts were characterized for thermal and organic solvents stabilities and compared with the soluble enzyme. The biocatalysts were then applied to the hydrolysis of the rac-indanyl acetate (2:1 ratio enzyme/substrate) at pH 7.0 and 30 °C for 24 h. For all the strategies evaluated, GEG promoted kinetic resolution of rac-indanyl acetate with maximum conversion (50%) and led to (R)-indanol with excellent enantiomeric excess (97%), maintaining the maximum conversion for five consecutive cycles of hydrolysis. Therefore, the use of cashew apple bagasse has proved to be a promising eco-friendly support for enzyme immobilization, since it resulted in stable biocatalysts for enzymatic kinetic resolution.
在这项研究中，通过使用固定在环保载体上的南极假丝酵母脂肪酶 B（用 10%缩水甘油基乙二胺-戊二醛（GEG）在不同的固定化策略（5 mM 或 100 mM 离子强度以及不存在或存在 0.5%（v/v）Triton X-100）下共价固定）来开发酶生物催化剂的调节。对生物催化剂进行了热稳定性和有机溶剂稳定性的特性研究，并与可溶性酶进行了比较。然后，将生物催化剂应用于 rac-茚满基乙酸酯（酶/底物比为 2:1）在 pH 7.0 和 30°C 下的水解 24 小时。在所评估的所有策略中，GEG 促进了 rac-茚满基乙酸酯的动力学拆分，转化率最高（50%），并得到了（R）-茚满醇，对映体过量值（ee）极高（97%），在五个连续的水解循环中保持了最大转化率。因此，证明了腰果苹果渣是一种很有前途的酶固定化环保载体，因为它得到了用于酶动力学拆分的稳定生物催化剂。
