Post-graduate Programm in Health Science, University of Southern Catarina (UNISUL), Tubarão, SC, Brazil; Laboratory of Experimental Neuroscience (LANEX)-UNISUL, Palhoça, SC, Brazil.
Pharmacy Degree Course UNISUL, Tubarão, SC, Brazil.
J Ethnopharmacol. 2021 Jan 10;264:113139. doi: 10.1016/j.jep.2020.113139. Epub 2020 Jul 26.
Inflammatory skin diseases presents high prevalence and lack of alternatives that can be used for self-care by the population. Casearia sylvestris is a plant used topically in different communities in Brazil, to treat wounds or promote cutaneous healing. To evaluate the topical anti-inflammatory activity for the crude hydroalcoholic extract of Casearia sylvestris (HCE-CS) in the models of single or multiple administration of chroton oil to induce ear edema in mice.
Experimental study using male Swiss mice (25-35g) kept under constant conditions in the Laboratory of Experimental Neuroscience (LaNEx)-UNISUL. Edema was induced in both models, respectively, by the single or multiple application of croton oil (CO, 2.5%, in 20 μl) on the external surface of the ear. The different groups of animals (n = 8) received different treatments: vehicle, dexamethasone (DEXA) or different doses of HCE-CS. Edema was evaluated macroscopically for 6 h (early edema) or 8 days (late edema) after the first application of the CO and immediately after the animals were submitted to euthanasia for the collection of the samples (treated ears). For early edema, the tissue was biochemically evaluated for myeloperoxidase activity (MPO) and levels of nitrite/nitrate. In the late edema model, the ears were histologically evaluated for general morphometry, degranulated and non-degranulated mast cells, as well as acanthosis.
Topic treatment with HCE-CS significantly reduced the early and late edema, as well as MPO activity and tissue levels of nitrite/nitrate. Finally, in the late edema model there was a lower density of degranulated mast cells in relation to the vehicle treated group and decreased thickness of the epidermis (acanthosis).
These results suggest a possible benefit of topical treatment with HCE-CS in inflammatory conditions of the skin.
炎症性皮肤病的发病率很高，而且缺乏可供民众自行护理的替代方法。巴西的不同社区都有将野桐树(Casearia sylvestris)用于治疗伤口或促进皮肤愈合的传统。为了评估野桐树粗醇提物（HCE-CS）在单次或多次涂抹巴豆油（诱导小鼠耳部水肿）模型中的局部抗炎活性。
这是一项在 UNISUL 实验神经科学实验室（LaNEx）中使用雄性瑞士小鼠（25-35g）进行的实验研究。两种模型均通过单次或多次应用巴豆油（CO，2.5%，20μl）于耳部外表面来诱导水肿。不同组别的动物（n=8）接受不同的处理：赋形剂、地塞米松（DEXA）或 HCE-CS 的不同剂量。在 CO 首次应用后 6 小时（早期水肿）或 8 天（晚期水肿）进行宏观评估，并在处死动物以收集样本（处理耳部）后立即进行。对于早期水肿，通过髓过氧化物酶活性（MPO）和亚硝酸盐/硝酸盐水平对组织进行生化评估。在晚期水肿模型中，对耳朵进行一般形态计量学、脱颗粒和未脱颗粒肥大细胞以及棘层肥厚的组织学评估。
HCE-CS 的局部治疗显著减轻了早期和晚期水肿，以及 MPO 活性和组织中硝酸盐/亚硝酸盐的水平。最后，在晚期水肿模型中，与赋形剂处理组相比，脱颗粒肥大细胞的密度降低，表皮厚度（棘层肥厚）减小。
这些结果表明，HCE-CS 的局部治疗可能对皮肤炎症有好处。
