Bouclier M, Shroot B, Milano G, Ferracin J, Chatelus A, Hensby C N
Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
Skin Pharmacol. 1988;1(1):24-9. doi: 10.1159/000210748.
Several biochemical parameters including ornithine decarboxylase activity (ODC) and tissue polyamine levels were measured during the hexadecane-induced epidermal hyperplasia of hairless rat skin. Animals received three applications of 200 microliters pure n-hexadecane on day 1. ODC activity and polyamine levels (putrescine, spermidine and spermine) in the epidermis were significantly increased and reached maximum elevations at 12 h after the start of n-hexadecane treatment with DNA synthesis peaking at 24 h. Histological studies confirmed a significant cellular edema at 24 h after the beginning of the treatment followed at 48 h by an epidermal hyperplasia which was maximum at 72 h. These data support the view that ODC activation, increased biosynthesis of polyamines and DNA are early events in epidermal cell hyperproliferation.
在十六烷诱导无毛大鼠皮肤表皮增生过程中，测定了包括鸟氨酸脱羧酶活性（ODC）和组织多胺水平在内的几个生化参数。动物在第1天接受三次200微升纯正十六烷的涂抹。表皮中的ODC活性和多胺水平（腐胺、亚精胺和精胺）显著增加，并在正十六烷处理开始后12小时达到最大升高，DNA合成在24小时达到峰值。组织学研究证实，处理开始后24小时出现明显的细胞水肿，48小时后出现表皮增生，72小时达到最大值。这些数据支持以下观点，即ODC激活、多胺生物合成增加和DNA合成是表皮细胞过度增殖的早期事件。
