The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, Shandong, 266237, China.
The State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, Shandong, 266237, China.
Enzyme Microb Technol. 2020 Sep;139:109568. doi: 10.1016/j.enzmictec.2020.109568. Epub 2020 Apr 15.
Galactokinases, which catalyze the phosphorylation of galactose and possible other monosaccharides, can provide an activated sugar donor to synthesize sugar-containing molecules. In this study, a novel galactokinase from human gut symbiont Akkermansia muciniphila ATCC BAA-835 (GalKAmu) was expressed and characterized. GalKAmu displayed broad substrate tolerance, with catalytic activity towards Gal (100 %), GalN (100 %), GalA (20.2 %), Glc (52.5 %), GlcNAc (15.5 %), Xyl (<5%), ManNAc (58 %), ManF (37.4 %) and l-Glc (80 %). Most interestingly, this was the first GalK isoform which can tolerate ManNAc. Thus, our characterization of GalKAmu broadens the substrate selection of galactokinases.
半乳糖激酶可催化半乳糖和可能其他单糖的磷酸化，可为合成糖缀合物分子提供活性糖供体。在这项研究中，我们从人类肠道共生菌阿克曼氏菌（Akkermansia muciniphila）ATCC BAA-835 中表达并鉴定了一种新型半乳糖激酶（GalKAmu）。GalKAmu 表现出广泛的底物耐受性，对 Gal（100%）、GalN（100%）、GalA（20.2%）、Glc（52.5%）、GlcNAc（15.5%）、Xyl（<5%）、ManNAc（58%）、ManF（37.4%）和 l-Glc（80%）具有催化活性。最有趣的是，这是第一个可以耐受 ManNAc 的 GalK 同工酶。因此，我们对半乳糖激酶 GalKAmu 的鉴定拓宽了半乳糖激酶的底物选择范围。
