König Christine, Plank Anne-Christine, Kapp Alexander, Timotius Ivanna K, von Hörsten Stephan, Zimmermann Katharina
Department of Anesthesiology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Department of Experimental Therapy, Preclinical Experimental Center, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Front Neurosci. 2020 Jul 7;14:531. doi: 10.3389/fnins.2020.00531. eCollection 2020.
We measured indirect calorimetry and activity parameters, VO and VCO to extract respiratory exchange ratio (RER) and energy expenditure in both sexes of 30 inbred mouse strains of 6 genetic families at 9-13 weeks during one photophase and the subsequent scotophase. We observed a continuous distribution of all traits. While males had higher body weights than females, we observed no sex difference for food and water intake. All strains drank and fed more during the night even if they displayed no day-night difference in activity traits. Several strains showed absent or weak day-night variation in one or more activity traits and these included FVB and 129X1, males of 129S1, SWR, NZW, and SM, and females of SJL. In general females showed higher rearing and ambulatory activity with 6 and 9 strains, respectively, showing a sex difference. Fine motor movements, like grooming, showed less sex differences. RER underlied a strong day-night difference and no sex effect. Only FVB females and males of the RIIIS and SM strain had no day-night variation. Energy expenditure underlies a large day-night variation which was absent in SWR and in FVB females and RIIIS males. In general, female bodies had a tendency to higher energy expenditure values, which became a significant difference in C3H, MAMy, SM, DBA1, and BUB. Our data illustrate the diversity of these traits in male and female inbred mice and provide a resource in the selection of strains for future studies.
我们测量了间接热量测定法和活动参数、耗氧量（VO）和二氧化碳排出量（VCO），以提取6个遗传家族的30个近交小鼠品系在9至13周龄时一个光照期及随后暗期内两性的呼吸交换率（RER）和能量消耗。我们观察到所有性状呈连续分布。虽然雄性小鼠的体重高于雌性，但我们观察到食物和水摄入量没有性别差异。即使所有品系在活动性状上没有昼夜差异，它们在夜间的饮水和进食量也更多。几个品系在一个或多个活动性状上没有或只有微弱的昼夜变化，这些品系包括FVB和129X1、129S1、SWR、NZW和SM品系的雄性，以及SJL品系的雌性。总体而言，分别有6个和9个品系的雌性表现出更高的竖毛和行走活动，显示出性别差异。像梳理毛发这样的精细运动，性别差异较小。RER存在强烈的昼夜差异且无性别效应。只有FVB品系的雌性以及RIIIS和SM品系中的雄性没有昼夜变化。能量消耗存在较大的昼夜变化，SWR品系以及FVB品系的雌性和RIIIS品系的雄性不存在这种变化。总体而言，雌性小鼠的能量消耗值有升高的趋势，在C3H、MAMy、SM、DBA1和BUB品系中这种差异变得显著。我们的数据说明了这些性状在近交雄性和雌性小鼠中的多样性，并为未来研究的品系选择提供了资源。
