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添加不同牛磺酸与蛋氨酸比例的全植物源蛋白质日粮对虹鳟幼鱼生长、巨噬细胞活性及抗氧化能力的影响

Effects of diets with whole plant-origin proteins added with different ratios of taurine:methionine on the growth, macrophage activity and antioxidant capacity of rainbow trout () fingerlings.

作者信息

Hernández Omar Ezequiel Aguillón, Hernández Luis Héctor Hernández, Miyasaka Armando Shimada, Lugo Mario Garduño

机构信息

Posgrado en Ciencias de la Producción y de la Salud Animal, Facultad de Estudios Superiores Cuatitlan UNAM, Edif. De Posgrado, Campo Uno, Av. Primero de Mayo S/N, Sta. Maria de las Torres, Cuatitlan Izcalli, Estado de México 54740, Mexico.

Laboratorio de Producción Acuícola, Facultad de Estudios Superiores Iztacala UNAM, Av. de los Barrios 1, Los Reyes Iztacala, Tlalnepantla, Estado de México 54090, Mexico.

出版信息

Vet Anim Sci. 2017 May 2;3:4-9. doi: 10.1016/j.vas.2017.04.002. eCollection 2017 Jun.

Abstract

A 70-days feeding trial was performed to determine the effect of diets with whole plant-origin proteins added with different ratios of taurine:methionine on the growth, macrophage burst activity and antioxidant capacity of rainbow trout () fingerlings. Triplicated groups of 70 fingerlings of an initial weight of 0.54±0.1 g (mean±±SD) were fed diets with soy protein isolate and powder as protein sources (46% crude protein) and added (10 g/kg diet) with different taurine and methionine ratios: 0.0 and 10.0 (diet T0/M100), 2.5 and 7.5 (diet T25/M75), 5.0 and 5.0 (diet T50/M50), 7.5 and 2.5 (diet T75/M25) and 10.0 and 0.0 (diet T100/M0), respectively. At the end of the trial, growth performance, lipid and protein contents in liver and muscle, macrophage burst activity and liver antioxidant activity, were determined. The growth performance, macrophage burst activity and antioxidant activity were improved as the taurine increased in the diets. The ratio of 7.5 and 2.5 g/kg of taurine:methionine in diets with SPI and powder as protein sources (diet T75/M25), seems to be the best inclusion for rainbow trout fingerlings.

摘要

进行了一项为期70天的饲养试验,以确定添加不同比例牛磺酸:蛋氨酸的全植物源蛋白质日粮对虹鳟()幼鱼生长、巨噬细胞爆发活性和抗氧化能力的影响。将初始体重为0.54±0.1 g(平均值±标准差)的70尾幼鱼分为三组,每组重复,分别投喂以大豆分离蛋白和 粉为蛋白质来源(粗蛋白含量46%)并添加(10 g/kg日粮)不同牛磺酸和蛋氨酸比例的日粮:0.0和10.0(日粮T0/M100)、2.5和7.5(日粮T25/M75)、5.0和5.0(日粮T50/M50)、7.5和2.5(日粮T75/M25)以及10.0和0.0(日粮T100/M0)。试验结束时,测定生长性能、肝脏和肌肉中的脂质和蛋白质含量、巨噬细胞爆发活性以及肝脏抗氧化活性。随着日粮中牛磺酸含量的增加,生长性能、巨噬细胞爆发活性和抗氧化活性均得到改善。以大豆分离蛋白和 粉为蛋白质来源的日粮中,牛磺酸:蛋氨酸比例为7.5和2.5 g/kg(日粮T75/M25)似乎是虹鳟幼鱼的最佳添加比例。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a50/7386729/a9e56e721bc0/gr1.jpg

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