Department of Ophthalmology, The Second Hospital of Hebei Medical University, Shijiazhaung, 050000, Hebei Province, China.
Moorfields Eye Hospitals UAE, Abu Dhabi, United Arab Emirates.
Int Ophthalmol. 2020 Dec;40(12):3413-3430. doi: 10.1007/s10792-020-01528-9. Epub 2020 Jul 30.
To identify novel tumor-specific features of ossification by using multispectral imaging (MSI) in patients diagnosed with choroidal osteoma.
Six eyes of 5 patients previously diagnosed with choroidal osteoma by ocular ultrasonography and orbital computerized tomography were observed with multispectral imaging (MSI). Traditional multimodal imaging, including color fundus photograph (CFP) and enhanced depth-imaging-optical coherence tomography (EDI-OCT), fundus autofluorescence (FAF), indocyanine green angiography/fundus fluorescein angiography (ICGA/FFA), was performed. Osseous features detected by MSI such as calcification and decalcification were characterized and compared with other imaging modalities.
In all 3 eyes with calcified choroidal osteoma (100%), MSI featured by the homogeneous reflectance in 550 nm but the beehive appearance in 600-680 nm and homogenous hyper-reflectance in 780-850 nm', indicating the compact bone in the outer layers and bone trabecula in the middle layer (Sandwich sign). The pigmentary change showed high agreement between MSI and FAF. In other 3 eyes with extensive decalcification, MSI was able to differentiate the inactive portion of the osteoma from the decalcified area. The inactive portion was characterized by geographic hyper-reflective islands with higher reflectivity border (floating island sign). Decalcified portion was featured by increased definition and reflectivity from osteoma. Partial decalcification and total decalcification can be differentiated in one decalcifying eye (33.3%). MSI revealed better the presence and border of the osteoma compared with FFA, FAF and MC (100%) in all six eyes in our study.
MSI presented characteristic osseous-related features of choroidal osteoma, providing clear evidence for differentiating osteoblastic and osteoclastic regions and noncalcifying regions. It can contribute to en-face visualization of choroidal osteomas at different stages, providing new insight into the spectrum behavior of CO.
通过对经眼部超声和眼眶计算机断层扫描诊断为脉络膜骨瘤的患者进行多光谱成像（MSI），来确定骨化的新的肿瘤特异性特征。
对 5 例经眼部超声和眼眶计算机断层扫描诊断为脉络膜骨瘤的患者的 6 只眼进行多光谱成像（MSI）观察。进行传统的多模态成像，包括彩色眼底照相（CFP）和增强深度成像-光学相干断层扫描（EDI-OCT）、眼底自发荧光（FAF）、吲哚菁绿血管造影/眼底荧光血管造影（ICGA/FFA）。对 MSI 检测到的骨化特征，如钙化和脱钙进行特征描述，并与其他成像方式进行比较。
在 3 只钙化性脉络膜骨瘤（100%）眼中，MSI 在 550nm 呈现均匀反射，而在 600-680nm 呈现蜂巢状外观，在 780-850nm 呈现均匀高反射，提示外层致密骨和中层骨小梁（三明治征）。色素变化在 MSI 和 FAF 之间具有很高的一致性。在另外 3 只广泛脱钙的眼中，MSI 能够区分骨瘤的非活动部分和脱钙区。非活动部分的特征是具有更高反射边界的点状高反射岛（漂浮岛征）。脱钙部分的特征是骨瘤的清晰度和反射率增加。在一只脱钙眼中（33.3%）可以区分部分脱钙和完全脱钙。在我们的研究中，MSI 在所有 6 只眼中均比 FFA、FAF 和 MC（100%）更好地显示了骨瘤的存在和边界。
MSI 呈现出脉络膜骨瘤与骨相关的特征性表现，为区分成骨细胞和破骨细胞区域及非钙化区域提供了明确证据。它可以有助于在不同阶段对脉络膜骨瘤进行全面可视化，为 CO 的谱行为提供新的见解。
