School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Curr Drug Metab. 2020;21(12):979-993. doi: 10.2174/1567201817666200731170731.
To study the compatibility regularity of Simiao Yong'an decoction by determining the plasma protein binding rate with the constituents in Simiao Yong'an decoction and to preliminarily clarify the effects of the compatibility on the plasma protein binding rate of different components.
Based on the equilibrium dialysis method, high-performance liquid chromatography was used to determine the contents of six constituents, which were divided into a single group and combination groups, in Simiao Yong'an decoction in the internal and external dialysis solutions. The obtained plasma protein binding rate through calculations was an index to evaluate the binding of the above components to plasma protein in different conditions.
Harpagide, harpagoside, sweroside and loganin showed low plasma protein binding rates, ferulic acid exhibited a moderate plasma protein binding rate, and glycyrrhizic acid showed a high plasma protein binding rate. The compatibility study showed that glycyrrhizic acid promoted the binding of ferulic acid to plasma protein. Glycyrrhizic acid and ferulic acid were the key compounds to promote the binding of harpagide to plasma protein. Glycyrrhizic acid, harpagide, harpagoside and loganin had a significant inhibitory effects on the binding of sweroside to plasma protein. The plasma protein binding capacities of harpagoside and loganin were reduced by the other five constituents. Glycyrrhizic acid had the strongest plasma protein binding effect, and the binding effect was not affected by other components.
This study explores the effects of compound compatibility on effective components from the perspective of plasma protein binding by high-performance liquid chromatography combined with the equilibrium dialysis method, and lays a foundation for clarifying the compatibility rule of Simiao Yong'an decoction and also provides a new idea for the study of the compatibility of traditional Chinese medicine formulas.
通过测定四妙勇安汤中各成分与血浆蛋白的结合率，研究四妙勇安汤的配伍规律，初步阐明配伍对各成分与血浆蛋白结合率的影响。
采用平衡透析法，用高效液相色谱法分别测定四妙勇安汤中 6 种成分在内外透析液中的含量，将其分为单一组和组合组，计算得到的血浆蛋白结合率作为评价上述各成分在不同条件下与血浆蛋白结合的指标。
哈巴俄苷、哈巴苷、獐牙菜苦苷和马钱苷表现出较低的血浆蛋白结合率，阿魏酸表现出中等的血浆蛋白结合率，甘草酸表现出较高的血浆蛋白结合率。配伍研究表明，甘草酸促进了阿魏酸与血浆蛋白的结合。甘草酸和阿魏酸是促进哈巴苷与血浆蛋白结合的关键化合物。甘草酸、哈巴苷、哈巴苷和马钱苷对獐牙菜苦苷与血浆蛋白的结合有显著抑制作用。獐牙菜苦苷和马钱苷的血浆蛋白结合能力降低。甘草酸对血浆蛋白的结合能力最强，且不受其他成分的影响。
本研究通过高效液相色谱结合平衡透析法，从血浆蛋白结合的角度探讨了复方配伍对有效成分的影响，为阐明四妙勇安汤的配伍规律奠定了基础，也为中药方剂的配伍研究提供了新的思路。
