Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
Curr Drug Deliv. 2020;17(9):806-814. doi: 10.2174/1567201817666200731164002.
This study aimed to investigate the existence of phospholipase-A (PLA) activity in Soluble L. major Antigens (SLA) because of no reports for it so far. Liposomes were used as sensors to evaluate PLA activity.
Liposomal SLA consisting of Egg Phosphatidylcholine (EPC) or Sphingomyelin (SM) were prepared by two different methods in different pH or temperatures and characterized by Dynamic Light Scattering (DLS) and Thin Layer Chromatography (TLC).
Lipid hydrolysis led to the disruption of EPC liposomal SLA in both methods but the Film Method (FM) produced more stable liposomes than the Detergent Removal Method (DRM).
The preparation of EPC liposomal SLA at pH 6 via FM protected liposomes from hydrolysis to some extent for a short time. EPC liposomes but not SM liposomes were disrupted in the presence of SLA.
Therefore, a phospholipid without ester bond such as SM should be utilized in liposome formulations containing PLA as an encapsulating protein.
本研究旨在调查可溶性 L. major 抗原 (SLA) 中是否存在磷脂酶-A (PLA) 活性，因为迄今为止尚无相关报道。脂质体被用作评估 PLA 活性的传感器。
通过两种不同的方法，在不同的 pH 值或温度下，用 Egg Phosphatidylcholine (EPC) 或 Sphingomyelin (SM) 制备脂质体 SLA，并通过动态光散射 (DLS) 和薄层层析 (TLC) 进行表征。
两种方法均导致 EPC 脂质体 SLA 的脂质水解，但薄膜法 (FM) 比去污剂去除法 (DRM) 产生更稳定的脂质体。
通过 FM 在 pH 6 下制备的 EPC 脂质体 SLA 在短时间内对水解有一定程度的保护作用。存在 SLA 时，EPC 脂质体而非 SM 脂质体被破坏。
因此，在含有 PLA 的脂质体制剂中，应使用不含酯键的磷脂，如 SM。
