Department of Biotechnology, Institute of Life Sciences, Nalco Square, Bhubaneswar, 751023, India.
Arch Microbiol. 2020 Dec;202(10):2761-2770. doi: 10.1007/s00203-020-01997-7. Epub 2020 Jul 31.
This study describes the community composition and functions of the gut microbiome of the freshwater omnivorous pufferfish based on metagenomic approach. Metagenome sequence data showed a dominance of the class Gammaproteobacteria followed by Fusobacteria, Actinobacteria, Anerolineae, Betaproteobacteria, Deinococci, Clostridia and Deltaproteobacteria. At the order level, the most abundant groups were Aeromonadales, Fusobacteriales, Enterobacterales, Synechococcales. The genus Aeromonas was the most predominant followed by Plesiomonas and Cetobacterium. Additionally, within the domain Archaea, class Methanomicrobia was most abundant followed by Hadesarchaea, Thermoplasmata, Candidatus Altiarchaeales, Candidatus Bathyarchaeota and Thermoprotei. The metabolic profile of the bacterial community exhibited a high prevalence of genes associated with core housekeeping functions, such as synthesis of cofactors, vitamins, prosthetic groups, pigments, amino acids and its derivatives, carbohydrate and protein metabolism. Comparative analysis with other fish gut microbiome showing similarity in protein metabolism with carnivorous Salmon and carbohydrate metabolism with herbivorous grass carp respectively. This study describes the bacterial community compositions are influenced by the trophic level.
本研究采用宏基因组学方法描述了淡水杂食性河豚鱼肠道微生物群落的组成和功能。宏基因组序列数据显示，γ-变形菌门（Gammaproteobacteria）占优势，其次为梭杆菌门（Fusobacteria）、放线菌门（Actinobacteria）、浮霉菌门（Anerolineae）、β-变形菌门（Betaproteobacteria）、厚壁菌门（Deinococci）、拟杆菌门（Clostridia）和δ-变形菌门（Deltaproteobacteria）。在目水平上，最丰富的类群是气单胞菌目（Aeromonadales）、梭杆菌目（Fusobacteriales）、肠杆菌目（Enterobacterales）、聚球藻目（Synechococcales）。气单胞菌属（Aeromonas）是最主要的属，其次是类志贺邻单胞菌属（Plesiomonas）和鲸杆菌属（Cetobacterium）。此外，在古菌域（Archaea）中，甲烷微菌纲（Methanomicrobia）最为丰富，其次是热变形菌门（Hadesarchaea）、泉古菌门（Thermoplasmata）、疑似古菌门（Candidatus Altiarchaeales）、疑似泉古菌门（Candidatus Bathyarchaeota）和暖层菌门（Thermoprotei）。细菌群落的代谢谱显示，与核心维持功能相关的基因高度流行，如辅酶、维生素、辅基、色素、氨基酸及其衍生物、碳水化合物和蛋白质代谢的合成。与其他鱼类肠道微生物组的比较分析表明，在蛋白质代谢方面与肉食性鲑鱼相似，在碳水化合物代谢方面与草食性草鱼相似。本研究描述了细菌群落组成受营养水平的影响。
