Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, Guangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.
Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, Guangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong Province, PR China.
Dev Comp Immunol. 2021 Jan;114:103801. doi: 10.1016/j.dci.2020.103801. Epub 2020 Jul 30.
The nuclear factor-κB (NF-κB) family is evolutionary conserved and plays key roles in the regulation of numerous basic cellular processes. In this study, a sea cucumber Holothuria leucospilota NF-κB1 p105 named HLp105 was first obtained. The full-length cDNA of HLp105 is 6564 bp long, with a 219 bp 5' untranslated region (UTR), a 2979 bp 3' UTR, and a 3366 bp open reading frame (ORF) encoding for 1121 amino acids with a deduced molecular weight of 123.92 kDa and an estimated pI of 5.31. HLp105 protein contains the conserved domain RHD, IPT, ANK and DEATH. HLp105 mRNA can be detected in all tissues examined, with the highest level in the intestine, followed by the transverse vessel, rete mirabile, coelomocytes, respiratory tree, bolishiti, cuvierian tubules, body wall, oesophagus and muscle. Challenged by LPS or poly (I:C), the transcription level of HLp105 was apparently up-regulated in the tissues examined. Besides, Over-expression of HLp105 in HEK293T cells, the apoptosis was inhibited, and the cytokines IL-1β and TNF-α were activated. The results are important for better understanding the function of NF-κB1 p105 in sea cucumber and reveal its involvement in immunoreaction.
核因子-κB（NF-κB）家族在进化上是保守的，在调节许多基本细胞过程中发挥着关键作用。在这项研究中，首次获得了一种海参糙海参 NF-κB1 p105，命名为 HLp105。HLp105 的全长 cDNA 长 6564 bp，具有 219 bp 的 5'非翻译区（UTR）、2979 bp 的 3'UTR 和 3366 bp 的开放阅读框（ORF），编码 1121 个氨基酸，推断分子量为 123.92 kDa，估计等电点为 5.31。HLp105 蛋白含有保守结构域 RHD、IPT、ANK 和 DEATH。HLp105 mRNA 可在所有检测组织中检测到，在肠中表达水平最高，其次是横血管、 rete mirabile、体腔细胞、呼吸树、bolishiti、cuvierian 管、体壁、食道和肌肉。用 LPS 或 poly（I:C）刺激后，检测到组织中 HLp105 的转录水平明显上调。此外，在 HEK293T 细胞中转染 HLp105 可抑制细胞凋亡，并激活细胞因子 IL-1β 和 TNF-α。这些结果对于更好地理解 NF-κB1 p105 在海参中的功能以及揭示其参与免疫反应具有重要意义。
