From the Department of Plastic Surgery and Hand Surgery-Burn Center and the Department of Anesthesiology, University Hospital RWTH Aachen; HBO-Center Euregio Aachen; and Aesthetic Elite International.
Plast Reconstr Surg. 2020 Aug;146(2):309-320. doi: 10.1097/PRS.0000000000007029.
Adipose-derived stem cells are considered as candidate cells for regenerative plastic surgery. Measures to influence cellular properties and thereby direct their regenerative potential remain elusive. Hyperbaric oxygen therapy-the exposure to 100% oxygen at an increased atmospheric pressure-has been propagated as a noninvasive treatment for a multitude of indications and presents a potential option to condition cells for tissue-engineering purposes. The present study evaluates the effect of hyperbaric oxygen therapy on human adipose-derived stem cells.
Human adipose-derived stem cells from healthy donors were treated with hyperbaric oxygen therapy at 2 and 3 atm. Viability before and after each hyperbaric oxygen therapy, proliferation, expression of surface markers and protein contents of transforming growth factor (TGF)-β, tumor necrosis factor-α, hepatocyte growth factor, and epithelial growth factor in the supernatants of treated adipose-derived stem cells were measured. Lastly, adipogenic, osteogenic, and chondrogenic differentiation with and without use of differentiation-inducing media (i.e., autodifferentiation) was examined.
Hyperbaric oxygen therapy with 3 atm increased viability, proliferation, and CD34 expression and reduced the CD31/CD34/CD45 adipose-derived stem cell subset and endothelial progenitor cell population. TGF-β levels were significantly decreased after two hyperbaric oxygen therapy sessions in the 2-atm group and decreased after three hyperbaric oxygen therapy sessions in the 3-atm group. Hepatocyte growth factor secretion remained unaltered in all groups. Although the osteogenic and chondrogenic differentiation were not influenced, adipogenic differentiation and autodifferentiation were significantly enhanced, with osteogenic autodifferentiation significantly alleviated by hyperbaric oxygen therapy with 3 atm.
Hyperbaric oxygen therapy with 3 atm increases viability and proliferation of adipose-derived stem cells, alters marker expression and subpopulations, decreases TGF-β secretion, and skews adipose-derived stem cells toward adipogenic differentiation.
CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, V.
脂肪来源的干细胞被认为是再生整形手术的候选细胞。影响细胞特性并因此指导其再生潜力的措施仍然难以捉摸。高压氧疗法 - 在增加的大气压下暴露于 100%氧气 - 已被宣传为多种适应症的非侵入性治疗方法，并为细胞的组织工程目的提供了一种潜在的选择。本研究评估了高压氧疗法对人脂肪来源干细胞的影响。
从健康供体中分离出人脂肪来源干细胞，用高压氧疗法在 2 和 3 个大气压下进行处理。在每次高压氧治疗前后测量细胞活力，增殖，表面标志物表达以及处理后脂肪来源干细胞上清液中转化生长因子（TGF）-β，肿瘤坏死因子-α，肝细胞生长因子和表皮生长因子的蛋白含量。最后，检查有无诱导分化培养基（即自动分化）的情况下的成脂，成骨和成软骨分化。
3 个大气压的高压氧治疗增加了细胞活力，增殖和 CD34 表达，并减少了 CD31/CD34/CD45 脂肪来源干细胞亚群和内皮祖细胞群。在 2 个大气压组中，两次高压氧治疗后 TGF-β水平显着降低，而在 3 个大气压组中，三次高压氧治疗后 TGF-β水平降低。所有组的肝细胞生长因子分泌均保持不变。尽管成骨和软骨分化不受影响，但成脂分化和自动分化显着增强，并且 3 个大气压的高压氧治疗显着减轻了成骨自动分化。
3 个大气压的高压氧治疗增加了脂肪来源干细胞的活力和增殖，改变了标志物表达和亚群，减少了 TGF-β分泌，并使脂肪来源干细胞向成脂分化倾斜。
临床问题/证据水平：治疗，V。
