Sugiyama Eiji, Sugiura Yuki
Department of Biochemistry, Keio University School of Medicine.
Yakugaku Zasshi. 2020;140(8):979-983. doi: 10.1248/yakushi.20-00012-3.
Monoamine neurotransmitters are released by specialized neurons that regulate behavioral and cognitive functions. Although localization of monoaminergic neurons in the brain is well known, the distribution, concentration, and kinetics of monoamines remain unclear. We used mass spectrometry imaging (MSI) for simultaneous and quantitative imaging of endogenous monoamines to generate a murine brain atlas of serotonin (5-HT), dopamine (DA), and norepinephrine (NE) levels. We observed several nuclei rich in both 5-HT and a catecholamine (DA or NE). Additionally, we analyzed de novo monoamine synthesis or fluctuations in those nuclei. We propose that MSI is a useful tool to gain deeper understanding of associations among the localization, levels, and turnover of monoamines in different brain areas and their role in inducing behavioral changes.
单胺类神经递质由调节行为和认知功能的特殊神经元释放。尽管大脑中单胺能神经元的定位已为人熟知，但单胺的分布、浓度和动力学仍不清楚。我们使用质谱成像（MSI）对内源性单胺进行同步定量成像，以生成小鼠大脑中血清素（5-HT）、多巴胺（DA）和去甲肾上腺素（NE）水平的图谱。我们观察到几个富含5-HT和一种儿茶酚胺（DA或NE）的核团。此外，我们分析了这些核团中的单胺从头合成或波动情况。我们认为，质谱成像（MSI）是一种有用的工具，有助于更深入地了解不同脑区中单胺的定位、水平和周转之间的关联及其在诱导行为变化中的作用。
